Anti-CEBPA Antibody (CAB0904)
- SKU:
- CAB0904
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-CEBPA Antibody |
| Antibody SKU: | CAB0904 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human CEBPA (NP_004355.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, Mouse kidney, Rat lung, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-205 of human CEBPA (NP_004355.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MESA DFYE AEPR PPMS SHLQ SPPH APSS AAFG FPRG AGPA QPPA PPAA PEPL GGIC EHET SIDI SAYI DPAA FNDE FLAD LFQH SRQQ EKAK AAVG PTGG GGGG DFDY PGAP AGPG GAVM PGGA HGPP PGYG CAAA GYLD GRLE PLYE RVGA PALR PLVI KQEP REED EAKQ LALA GLFP YQPP PPPP PSHP HPHP PPAH LAAP H |
| Gene ID: | 1050 |
| Uniprot: | P49715 |
| Cellular Location: | Nucleus, Nucleus, nucleolus |
| Calculated MW: | 25kDa/35kDa/37kDa/41kDa |
| Observed MW: | 30KDa/42KDa |
| Synonyms: | CEBPA, C/EBP-alpha, CEBP |
| Background: | This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. |
| UniProt Protein Function: | C/EBP-alpha: a bZIP transcription factor which can form homodimers or heterodimers with the related proteins CEBP-beta and CEBP-gamma. Binds to the promoter and modulate the expression of the gene encoding leptin, a protein that plays an important role in body weight homeostasis. Can interact with CDK2 and CDK4, thereby inhibiting these kinases and causing growth arrest in cultured cells. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 19q13.1 Cellular Component: Rb-E2F complex; nuclear matrix; nucleus Molecular Function:RNA polymerase II transcription factor activity, enhancer binding; protein binding; protein homodimerization activity; DNA binding; protein heterodimerization activity; protein complex binding; transcription factor activity; transcription factor binding Biological Process: transcription from RNA polymerase II promoter; fat cell differentiation; embryonic placenta development; macrophage differentiation; viral reproduction; cell maturation; response to glucocorticoid stimulus; negative regulation of transcription from RNA polymerase II promoter; glucose homeostasis; negative regulation of cell proliferation; response to vitamin B2; acute-phase response; inner ear development; cholesterol metabolic process; mitochondrion organization and biogenesis; generation of precursor metabolites and energy; organ regeneration; granulocyte differentiation; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; negative regulation of cyclin-dependent protein kinase activity; liver development; positive regulation of osteoblast differentiation; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; white fat cell differentiation; brown fat cell differentiation; positive regulation of fat cell differentiation; positive regulation of transcription from RNA polymerase III promoter; myeloid cell differentiation; positive regulation of transcription from RNA polymerase II promoter; urea cycle; lung development Disease: Leukemia, Acute Myeloid |
| NCBI Summary: | This intronless gene encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of this gene is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons. [provided by RefSeq, Dec 2013] |
| UniProt Code: | P49715 |
| NCBI GenInfo Identifier: | 166898082 |
| NCBI Gene ID: | 1050 |
| NCBI Accession: | P49715.3 |
| UniProt Secondary Accession: | P49715,P78319, Q05CA4, A7LNP2, |
| UniProt Related Accession: | P49715 |
| Molecular Weight: | 37,561 Da |
| NCBI Full Name: | CCAAT/enhancer-binding protein alpha |
| NCBI Synonym Full Names: | CCAAT/enhancer binding protein (C/EBP), alpha |
| NCBI Official Symbol: | CEBPA |
| NCBI Official Synonym Symbols: | CEBP; C/EBP-alpha |
| NCBI Protein Information: | CCAAT/enhancer-binding protein alpha |
| UniProt Protein Name: | CCAAT/enhancer-binding protein alpha |
| Protein Family: | CCAAT/enhancer-binding protein |
| UniProt Gene Name: | CEBPA |
| UniProt Entry Name: | CEBPA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CEBPA antibody ( A0904) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded rat liver using CEBPA Rabbit pAb (CAB0904) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophageal cancer using CEBPA Rabbit pAb (CAB0904) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using CEBPA Rabbit pAb (CAB0904) at dilution of 1:100 (40x lens). |