Anti-CDKN2B 항체 (CAB5685)

Antibody Name:	Anti-CDKN2B Antibody
Antibody SKU:	CAB5685
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human CDKN2B (NP_004927.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	HeLa, SW620, A-549

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-138 of human CDKN2B (NP_004927.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MREE NKGM PSGG GSDE GLAS AAAR GLVE KVRQ LLEA GADP NGVN RFGR RAIQ VMMM GSAR VAEL LLLH GAEP NCAD PATL TRPV HDAA REGF LDTL VVLH RAGA RLDV RDAW GRLP VDLA EERG HRDV AGYL RTAT GD
Gene ID:	1030
Uniprot:	P42772
Cellular Location:	Cytoplasm
Calculated MW:	8kDa/14kDa
Observed MW:	15kDa

Synonyms:

CDKN2B, CDK4I, INK4B, MTS2, P15, TP15, p15INK4b

Background:

This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported.

UniProt Protein Function:	p15-INK4B: a potent inhibitor that nteracts strongly with CDK4 and CDK6. Potential effector of TGF-beta induced cell cycle arrest.
UniProt Protein Details:	Protein type:Tumor suppressor; Protein kinase, regulatory subunit; Cell cycle regulation; Inhibitor Chromosomal Location of Human Ortholog: 9p21 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:cyclin-dependent protein kinase inhibitor activity; protein binding; protein kinase binding Biological Process: cell cycle arrest; cellular response to extracellular stimulus; cellular response to nutrient; G2/M transition of mitotic cell cycle; megakaryocyte differentiation; mitotic cell cycle checkpoint; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transforming growth factor beta receptor signaling pathway; regulation of cyclin-dependent protein kinase activity
NCBI Summary:	This gene lies adjacent to the tumor suppressor gene CDKN2A in a region that is frequently mutated and deleted in a wide variety of tumors. This gene encodes a cyclin-dependent kinase inhibitor, which forms a complex with CDK4 or CDK6, and prevents the activation of the CDK kinases, thus the encoded protein functions as a cell growth regulator that controls cell cycle G1 progression. The expression of this gene was found to be dramatically induced by TGF beta, which suggested its role in the TGF beta induced growth inhibition. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. [provided by RefSeq, Jul 2008]
UniProt Code:	P42772
NCBI GenInfo Identifier:	1168869
NCBI Gene ID:	1030
NCBI Accession:	P42772.1
UniProt Secondary Accession:	P42772,O15125, Q6FI09,
UniProt Related Accession:	P42772,AAB32712
Molecular Weight:	8,078 Da
NCBI Full Name:	Cyclin-dependent kinase 4 inhibitor B
NCBI Synonym Full Names:	cyclin dependent kinase inhibitor 2B
NCBI Official Symbol:	CDKN2B
NCBI Official Synonym Symbols:	P15; MTS2; TP15; CDK4I; INK4B; p15INK4b
NCBI Protein Information:	cyclin-dependent kinase 4 inhibitor B
UniProt Protein Name:	Cyclin-dependent kinase 4 inhibitor B
UniProt Synonym Protein Names:	Multiple tumor suppressor 2; MTS-2; p14-INK4b; p15-INK4b; p15INK4B
Protein Family:	Cyclin-dependent kinase 4 inhibitor
UniProt Gene Name:	CDKN2B
UniProt Entry Name:	CDN2B_HUMAN

Western blot analysis of extracts of various cell lines, using CDKN2B antibody (CAB5685) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

Antibodies

CDKN2B Antibody (PACO01277)

CDKN2B Antibody (PACO08348)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)