Anti-CDK2AP1 Antibody (CAB19985)[KO Validated]

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SKU:
CAB19985
Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Cycle
Frequently bought together:

Description

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Antibody Name:Anti-CDK2AP1 Antibody [KO Validated]
Antibody SKU:CAB19985
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant protein of human CDK2AP1.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:293T
Immunogen:Recombinant protein of human CDK2AP1.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:8099
Uniprot:O14519
Cellular Location:
Calculated MW:9kDa/12kDa
Observed MW:12KDa
Anti-CDK2AP1 Antibody [KO Validated] (CAB19985)Western blot analysis of extracts from normal (control) and CDK2AP1 knockout (KO) 293T cells, using CDK2AP1 antibody (CAB19985) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Synonyms:CDK2AP1, DOC1, DORC1, ST19, doc-1, p12DOC-1
Background:The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
UniProt Protein Function:CDK2AP1: specific inhibitor of the cell-cycle kinase CDK2. Belongs to the CDK2AP family.
UniProt Protein Details:

Protein type:DNA replication; Tumor suppressor; Cell cycle regulation

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: perinuclear region of cytoplasm; nucleus

Biological Process: positive regulation of protein amino acid phosphorylation; DNA-dependent DNA replication; cell cycle

NCBI Summary:The protein encoded by this gene is a cyclin-dependent kinase 2 (CDK2) -associated protein which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggests a regulatory role in DNA replication during the S-phase of the cell cycle. This protein also forms a core subunit of the nucleosome remodeling and histone deacetylation (NURD) complex that epigenetically regulates embryonic stem cell differentiation. This gene thus plays a role in both cell-cycle and epigenetic regulation. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2012]
UniProt Code:O14519
NCBI GenInfo Identifier:6014988
NCBI Gene ID:8099
NCBI Accession:O14519.1
UniProt Secondary Accession:O14519,F5GYA4,
UniProt Related Accession:O14519
Molecular Weight:115
NCBI Full Name:Cyclin-dependent kinase 2-associated protein 1
NCBI Synonym Full Names:cyclin-dependent kinase 2 associated protein 1
NCBI Official Symbol:CDK2AP1  
NCBI Official Synonym Symbols:DOC1; ST19; DORC1; doc-1; p12DOC-1  
NCBI Protein Information:cyclin-dependent kinase 2-associated protein 1; Deleted in oral cancer-1; deleted in oral cancer 1; CDK2-associated protein 1; putative oral cancer suppressor
UniProt Protein Name:Cyclin-dependent kinase 2-associated protein 1
UniProt Synonym Protein Names:Deleted in oral cancer 1; DOC-1; Putative oral cancer suppressor
Protein Family:Cyclin-dependent kinase 2-associated protein
UniProt Gene Name:CDK2AP1  
UniProt Entry Name:CDKA1_HUMAN

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