Anti-CDC25C Antibody (CAB1672)
- SKU:
- CAB1672
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-CDC25C Antibody |
| Antibody SKU: | CAB1672 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 174-473 of human CDC25C (NP_001781.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | K-562, HepG2, Raji |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 174-473 of human CDC25C (NP_001781.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PKLD KNPN LGED QAEE ISDE LMEF SLKD QEAK VSRS GLYR SPSM PENL NRPR LKQV EKFK DNTI PDKV KKKY FSGQ GKLR KGLC LKKT VSLC DITI TQML EEDS NQGH LIGD FSKV CALP TVSG KHQD LKYV NPET VAAL LSGK FQGL IEKF YVID CRYP YEYL GGHI QGAL NLYS QEEL FNFF LKKP IVPL DTQK RIII VFHC EFSS ERGP RMCR CLRE EDRS LNQY PALY YPEL YILK GGYR DFFP EYME LCEP QSYC PMHH QDHK TELL RCRS QSKV QEGE RQLR EQIA LLVK DMSP |
| Gene ID: | 995 |
| Uniprot: | P30307 |
| Cellular Location: | Nucleus |
| Calculated MW: | 45kDa/48kDa/50kDa/53kDa |
| Observed MW: | 70kDa |
| Synonyms: | CDC25C, CDC25, PPP1R60 |
| Background: | This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. |
| UniProt Protein Function: | CDC25C: a member of the MPI phosphatase family. Activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. Shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. At least four transcript variants for this gene exist. Three splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity Chromosomal Location of Human Ortholog: 5q31 Cellular Component: cytosol; nucleoplasm; nucleus Molecular Function:phosphoprotein phosphatase activity; protein binding; protein kinase binding; WW domain binding Biological Process: cell proliferation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA replication; G2/M transition of mitotic cell cycle; regulation of cell cycle; regulation of cyclin-dependent protein kinase activity; regulation of mitosis |
| NCBI Summary: | This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015] |
| UniProt Code: | P30307 |
| NCBI GenInfo Identifier: | 116242631 |
| NCBI Gene ID: | 995 |
| NCBI Accession: | P30307.2 |
| UniProt Secondary Accession: | P30307,Q96PL3, Q9H168, Q9H2E8, Q9H2E9, Q9H2F1, D3DQB8 |
| UniProt Related Accession: | P30307 |
| Molecular Weight: | 45,608 Da |
| NCBI Full Name: | M-phase inducer phosphatase 3 |
| NCBI Synonym Full Names: | cell division cycle 25C |
| NCBI Official Symbol: | CDC25C |
| NCBI Official Synonym Symbols: | CDC25; PPP1R60 |
| NCBI Protein Information: | M-phase inducer phosphatase 3 |
| UniProt Protein Name: | M-phase inducer phosphatase 3 |
| UniProt Synonym Protein Names: | Dual specificity phosphatase Cdc25C |
| UniProt Gene Name: | CDC25C |
| UniProt Entry Name: | MPIP3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDC25C antibody (CAB1672) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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