Anti-CDC25A Antibody (CAB1173)
- SKU:
- CAB1173
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-CDC25A Antibody |
| Antibody SKU: | CAB1173 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 235-484 of human CDC25A (NP_963861.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, K562 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 235-484 of human CDC25A (NP_963861.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RPER SQEE SPPG STKR RKSM SGAS PKES TNPE KAHE TLHQ SLSL ASSP KGTI ENIL DNDP RDLI GDFS KGYL FHTV AGKH QDLK YISP EIMA SVLN GKFA NLIK EFVI IDCR YPYE YEGG HIKG AVNL HMEE EVED FLLK KPIV PTDG KRVI VVFH CEFS SERG PRMC RYVR ERDR LGNE YPKL HYPE LYVL KGGY KEFF MKCQ SYCE PPSY RPMH HEDF KEDL KKFR TKSR TWAG EKSK REMY SRLK KL |
| Gene ID: | 993 |
| Uniprot: | P30304 |
| Cellular Location: | |
| Calculated MW: | 54kDa/59kDa |
| Observed MW: | 59kDa |
| Synonyms: | CDC25A, CDC25A2 |
| Background: | CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CDC25A: a member of the MPI phosphatase family. Functions as a dosage-dependent inducer in mitotic control. Required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two splice variant isoforms have been described. |
| UniProt Protein Details: | Protein type:Protein phosphatase, dual-specificity; EC 3.1.3.48; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 3p21 Cellular Component: nucleoplasm; cytosol Molecular Function:protein binding; protein tyrosine phosphatase activity; protein kinase binding Biological Process: cell proliferation; mitosis; response to radiation; cell division; regulation of cell cycle; mitotic cell cycle; regulation of cyclin-dependent protein kinase activity; DNA replication; G2/M transition of mitotic cell cycle; G1/S transition of mitotic cell cycle |
| NCBI Summary: | CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P30304 |
| NCBI GenInfo Identifier: | 50403734 |
| NCBI Gene ID: | 993 |
| NCBI Accession: | P30304.2 |
| UniProt Secondary Accession: | P30304,Q8IZH5, Q96IL3, Q9H2F2, |
| UniProt Related Accession: | P30304 |
| Molecular Weight: | |
| NCBI Full Name: | M-phase inducer phosphatase 1 |
| NCBI Synonym Full Names: | cell division cycle 25A |
| NCBI Official Symbol: | CDC25A |
| NCBI Official Synonym Symbols: | CDC25A2 |
| NCBI Protein Information: | M-phase inducer phosphatase 1; CDC25A2-CAG isoform; CDC25 isoform A1-CAG; dual specificity phosphatase CDC25A |
| UniProt Protein Name: | M-phase inducer phosphatase 1 |
| UniProt Synonym Protein Names: | Dual specificity phosphatase Cdc25A |
| Protein Family: | M-phase inducer phosphatase |
| UniProt Gene Name: | CDC25A |
| UniProt Entry Name: | MPIP1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CDC25A antibody (CAB1173) at 1:800 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat liver using CDC25A antibody (CAB1173) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver damage using CDC25A antibody (CAB1173) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using CDC25A antibody (CAB1173) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using CDC25A Rabbit pAb (CAB1173) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |