Anti-CDC20 항체 (CAB1231)

Antibody Name:	Anti-CDC20 Antibody
Antibody SKU:	CAB1231
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CDC20 (NP_001246.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	U-251MG, DU145, SW480, THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human CDC20 (NP_001246.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAQF AFES DLHS LLQL DAPI PNAP PARW QRKA KEAA GPAP SPMR AANR SHSA GRTP GRTP GKSS SKVQ TTPS KPGG DRYI PHRS AAQM EVAS FLLS KENQ PENS QTPT KKEH QKAW ALNL NGFD VEEA KILR LSGK PQNA PEGY QNRL KVLY SQKA TPGS SRKT CRYI PSLP DRIL DAPE IRND YYLN LVDW SSGN VLAV ALDN SVYL WSAS SGDI LQLL QMEQ PGEY ISSV AWIK EGNY LAVG TSSA EVQL WDVQ QQKR LRNM TSHS AR
Gene ID:	991
Uniprot:	Q12834
Cellular Location:	Cytoplasm, centrosome, cytoskeleton, microtubule organizing center, spindle pole
Calculated MW:	54kDa
Observed MW:	55kDa

Synonyms:	CDC20, CDC20A, bA276H19.3, p55CDC
Background:	CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation.

UniProt Protein Function:	CDC20: Required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. Is regulated by MAD2L1: in metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The CDC20-APC/C complex positively regulates the formation of synaptic vesicle clustering at active zone to the presynaptic membrane in postmitotic neurons. CDC20-APC/C-induced degradation of NEUROD2 induces presynaptic differentiation. Found in a complex with CDC20, CDC27, SPATC1 and TUBG1. Interacts with SPATC1. Interacts with NEUROD2. Interacts with MAD2L1 and BUB1B. The phosphorylated form interacts with APC/C. Interacts with NINL. May interact with MAD2L2. Interacts with CDK5RAP2. Interacts with isoform 1 of NEK2. Belongs to the WD repeat CDC20/Fizzy family.
UniProt Protein Details:	Protein type:Ubiquitin conjugating system; Cell cycle regulation Chromosomal Location of Human Ortholog: 1p34.1 Cellular Component: anaphase-promoting complex; cytoplasm; cytosol; nucleoplasm; nucleus; spindle Molecular Function:enzyme binding; protein binding; protein C-terminus binding Biological Process: anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; positive regulation of synaptic plasticity; positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; sister chromatid cohesion
NCBI Summary:	CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. [provided by RefSeq, Jul 2008]
UniProt Code:	Q12834
NCBI GenInfo Identifier:	37537762
NCBI Gene ID:	991
NCBI Accession:	Q12834.2
UniProt Secondary Accession:	Q12834,Q5JUY4, Q9BW56, Q9UQI9, B2R6Z6, D3DPJ1,
UniProt Related Accession:	Q12834
Molecular Weight:	54,723 Da
NCBI Full Name:	Cell division cycle protein 20 homolog
NCBI Synonym Full Names:	cell division cycle 20
NCBI Official Symbol:	CDC20
NCBI Official Synonym Symbols:	CDC20A; p55CDC; bA276H19.3
NCBI Protein Information:	cell division cycle protein 20 homolog
UniProt Protein Name:	Cell division cycle protein 20 homolog
UniProt Synonym Protein Names:	p55CDC
Protein Family:	Cell division cycle protein
UniProt Gene Name:	CDC20
UniProt Entry Name:	CDC20_HUMAN

Western blot analysis of extracts of various cell lines, using CDC20 antibody (CAB1231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Antibodies

Anti-CDC20 Antibody (CAB15656)

CDC20 Antibody (PACO06334)

CDC20 Antibody (PACO08309)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)