Anti-CD40L Antibody (CAB0327)

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SKU:
CAB0327
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-CD40L Antibody
Antibody SKU:CAB0327
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 47-223 of human CD40L (NP_000065.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:Mouse lung, Mouse liver
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 47-223 of human CD40L (NP_000065.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HRRL DKIE DERN LHED FVFM KTIQ RCNT GERS LSLL NCEE IKSQ FEGF VKDI MLNK EETK KENS FEMQ KGDQ NPQI AAHV ISEA SSKT TSVL QWAE KGYY TMSN NLVT LENG KQLT VKRQ GLYY IYAQ VTFC SNRE ASSQ APFI ASLC LKSP GRFE RILL RAAN THSS AKPC GQQS I
Gene ID:959
Uniprot:P29965
Cellular Location:Cell membrane, Secreted, Single-pass type II membrane protein
Calculated MW:29kDa
Observed MW:30kDa
Synonyms:CD154, CD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, gp39, hCD40L, CD40LG
Background:The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome.
UniProt Protein Function:CD40LG: Mediates B-cell proliferation in the absence of co- stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching. Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1); also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. Belongs to the tumor necrosis factor family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: Xq26

Cellular Component: extracellular space; integral to plasma membrane; integral to membrane; plasma membrane; external side of plasma membrane

Molecular Function:CD40 receptor binding; cytokine activity; tumor necrosis factor receptor binding

Biological Process: B cell proliferation; platelet activation; regulation of immune response; leukocyte adhesion; positive regulation of interleukin-12 production; B cell differentiation; immunoglobulin secretion; isotype switching; inflammatory response; regulation of immunoglobulin secretion; negative regulation of apoptosis

Disease: Immunodeficiency With Hyper-igm, Type 1

NCBI Summary:The protein encoded by this gene is expressed on the surface of T cells. It regulates B cell function by engaging CD40 on the B cell surface. A defect in this gene results in an inability to undergo immunoglobulin class switch and is associated with hyper-IgM syndrome. [provided by RefSeq, Jul 2008]
UniProt Code:P29965
NCBI GenInfo Identifier:231718
NCBI Gene ID:959
NCBI Accession:P29965.1
UniProt Related Accession:P29965
Molecular Weight:29,274 Da
NCBI Full Name:CD40 ligand
NCBI Synonym Full Names:CD40 ligand
NCBI Official Symbol:CD40LG  
NCBI Official Synonym Symbols:IGM; IMD3; TRAP; gp39; CD154; CD40L; HIGM1; T-BAM; TNFSF5; hCD40L  
NCBI Protein Information:CD40 ligand; CD40-L; CD40 antigen ligand; T-cell antigen Gp39; T-B cell-activating molecule; TNF-related activation protein; tumor necrosis factor (ligand) superfamily member 5
UniProt Protein Name:CD40 ligand
UniProt Synonym Protein Names:T-cell antigen Gp39; TNF-related activation protein; TRAP; Tumor necrosis factor ligand superfamily member 5; CD_antigen: CD154Cleaved into the following 2 chains:CD40 ligand, membrane form; CD40 ligand, soluble form
UniProt Gene Name:CD40LG  
UniProt Entry Name:CD40L_HUMAN
Anti-CD40L Antibody (CAB0327)Western blot analysis of extracts of various cell lines, using CD40L antibody (CAB0327) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Anti-CD40L Antibody (CAB0327)Immunofluorescence analysis of U2OS cells using CD40L antibody (CAB0327). Blue: DAPI for nuclear staining.

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