Anti-CD4 Antibody (CAB1549)
- SKU:
- CAB1549
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-CD4 Antibody |
| Antibody SKU: | CAB1549 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human CD4 (NP_000607.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:1000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | SW620, Mouse brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 300-400 of human CD4 (NP_000607.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ALEA KTGK LHQE VNLV VMRA TQLQ KNLT CEVW GPTS PKLM LSLK LENK EAKV SKRE KAVW VLNP EAGM WQCL LSDS GQVL LESN IKVL PTWS TPVQ PMAL I |
| Gene ID: | 920 |
| Uniprot: | P01730 |
| Cellular Location: | Cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 51kDa |
| Observed MW: | 55kDa |
| Synonyms: | CD4, CD4mut |
| Background: | This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. |
| UniProt Protein Function: | CD4: Accessory protein for MHC class-II antigen/T-cell receptor interaction. May regulate T-cell activation. Induces the aggregation of lipid rafts. Associates with LCK. Binds to HIV-1 gp120 and to P4HB/PDI and upon HIV-1 binding to the cell membrane, is part of P4HB/PDI- CD4-CXCR4-gp120 complex. Interacts with HIV-1 Envelope polyprotein gp160 and protein Vpu. Interacts with Human Herpes virus 7 capsid proteins. Interacts with PTK2/FAK1; this interaction requires the presence of HIV-1 gp120. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Cell surface Chromosomal Location of Human Ortholog: 12p13.31 Cellular Component: T cell receptor complex; endoplasmic reticulum membrane; endoplasmic reticulum lumen; early endosome; plasma membrane; integral to membrane; external side of plasma membrane; lipid raft Molecular Function:protein binding; enzyme binding; transmembrane receptor activity; protein homodimerization activity; zinc ion binding; extracellular matrix structural constituent; coreceptor activity; receptor activity; glycoprotein binding; protein kinase binding; MHC class II protein binding Biological Process: maintenance of cellular protein localization; viral reproduction; positive regulation of interleukin-2 biosynthetic process; cytokine production; T cell selection; positive regulation of calcium-mediated signaling; defense response to Gram-negative bacterium; signal transduction; induction by virus of cell-cell fusion in host; T cell receptor signaling pathway; enzyme linked receptor protein signaling pathway; regulation of defense response to virus by virus; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; positive regulation of protein kinase activity; T cell costimulation; protein palmitoleylation; innate immune response; entry into host cell; immune response; transmembrane receptor protein tyrosine kinase signaling pathway; T cell differentiation; regulation of T cell activation Disease: Okt4 Epitope Deficiency |
| NCBI Summary: | This gene encodes a membrane glycoprotein of T lymphocytes that interacts with major histocompatibility complex class II antigenes and is also a receptor for the human immunodeficiency virus. This gene is expressed not only in T lymphocytes, but also in B cells, macrophages, and granulocytes. It is also expressed in specific regions of the brain. The protein functions to initiate or augment the early phase of T-cell activation, and may function as an important mediator of indirect neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Aug 2010] |
| UniProt Code: | P01730 |
| NCBI GenInfo Identifier: | 116013 |
| NCBI Gene ID: | 920 |
| NCBI Accession: | P01730.1 |
| UniProt Secondary Accession: | P01730,Q4ZGK2, Q5U066, Q9UDE5, B2R737, D3DUS5, |
| UniProt Related Accession: | P01730 |
| Molecular Weight: | 458 |
| NCBI Full Name: | T-cell surface glycoprotein CD4 |
| NCBI Synonym Full Names: | CD4 molecule |
| NCBI Official Symbol: | CD4 |
| NCBI Official Synonym Symbols: | CD4mut |
| NCBI Protein Information: | T-cell surface glycoprotein CD4; CD4 receptor; CD4 antigen (p55); T-cell surface antigen T4/Leu-3 |
| UniProt Protein Name: | T-cell surface glycoprotein CD4 |
| UniProt Synonym Protein Names: | T-cell surface antigen T4/Leu-3; CD_antigen: CD4 |
| Protein Family: | CD40 ligand |
| UniProt Gene Name: | CD4 |
| UniProt Entry Name: | CD4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CD4 antibody (CAB1549) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
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