Anti-CD3EAP Antibody (CAB16099)
- SKU:
- CAB16099
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Frequently bought together:
Description
| Antibody Name: | Anti-CD3EAP Antibody |
| Antibody SKU: | CAB16099 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human CD3EAP (NP_036231.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HeLa, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human CD3EAP (NP_036231.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEEP QAGD AARF SCPP NFTA KPPA SESP RFSL EALT GPDT ELWL IQAP ADFA PECF NGRH VPLS GSQI VKGK LAGK RHRY RVLS SCPQ AGEA TLLA PSTE AGGG LTCA SAPQ GTLR ILEG PQQS LSGS PLQP IPAS PPPQ IPPG LRPR FCAF GGNP PVTG PRSA LAPN LL |
| Gene ID: | 10849 |
| Uniprot: | O15446 |
| Cellular Location: | Chromosome, Nucleus, nucleolus |
| Calculated MW: | 54kDa/55kDa |
| Observed MW: | 70kDa |
| Synonyms: | CD3EAP, ASE-1, ASE1, CAST, PAF49 |
| Background: |
| UniProt Protein Function: | ASE-1: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Isoform 1 is involved in UBTF-activated transcription, presumably at a step following PIC formation. Belongs to the eukaryotic RPA34 RNA polymerase subunit family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nucleolus; Transcription initiation complex Chromosomal Location of Human Ortholog: 19q13.3 Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleolus; RNA polymerase I transcription factor complex; chromosome; nucleus; DNA-directed RNA polymerase I complex Molecular Function:DNA-directed RNA polymerase activity Biological Process: negative regulation of gene expression, epigenetic; RNA elongation from RNA polymerase I promoter; transcription from RNA polymerase I promoter; gene expression; transcription initiation from RNA polymerase I promoter; termination of RNA polymerase I transcription; regulation of gene expression, epigenetic; transmembrane receptor protein tyrosine kinase signaling pathway; rRNA transcription |
| UniProt Code: | O15446 |
| NCBI GenInfo Identifier: | 6912246 |
| NCBI Gene ID: | 10849 |
| NCBI Accession: | NP_036231.1 |
| UniProt Secondary Accession: | O15446,Q32N11, Q7Z5U2, Q9UPF6, |
| UniProt Related Accession: | O15446 |
| Molecular Weight: | 54,986 Da |
| NCBI Full Name: | DNA-directed RNA polymerase I subunit RPA34 |
| NCBI Synonym Full Names: | CD3e molecule, epsilon associated protein |
| NCBI Official Symbol: | CD3EAP |
| NCBI Official Synonym Symbols: | ASE1; CAST; ASE-1; PAF49 |
| NCBI Protein Information: | DNA-directed RNA polymerase I subunit RPA34; A34.5; antisense to ERCC 1; CD3E-associated protein; antisense to ERCC-1 protein; CD3 epsilon associated protein; CD3-epsilon-associated protein; RNA polymerase I-associated factor PAF49; CD3e antigen, epsilon |
| UniProt Protein Name: | DNA-directed RNA polymerase I subunit RPA34 |
| UniProt Synonym Protein Names: | A34.5; Antisense to ERCC-1 protein; ASE-1; CD3-epsilon-associated protein; CAST; CD3E-associated protein; RNA polymerase I-associated factor PAF49 |
| Protein Family: | Calpastatin |
| UniProt Gene Name: | CD3EAP |
| UniProt Entry Name: | RPA34_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CD3EAP antibody (CAB16099) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
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