Anti-CD3E Antigen Antibody (CAB12415)
- SKU:
- CAB12415
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-CD3E Antigen Antibody |
| Antibody SKU: | CAB12415 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human CD3E Antigen (NP_000724.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, Raji, MCF7, Rat thymus |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100 to the C-terminus of human CD3E Antigen (NP_000724.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PRGS KPED ANFY LYLR ARVC ENCM EMDV MSVA TIVI VDIC ITGG LLLL VYYW SKNR KAKA KPVT RGAG AGGR QRGQ NKER PPPV PNPD YEPI RKGQ RDLY SGLN QRRI |
| Gene ID: | 916 |
| Uniprot: | P07766 |
| Cellular Location: | Cell membrane, Single-pass type I membrane protein |
| Calculated MW: | 23kDa |
| Observed MW: | 23-25kDa |
| Synonyms: | CD3E, IMD18, T3E, TCRE, CD3e molecule |
| Background: | The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. |
| UniProt Protein Function: | CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain. |
| UniProt Protein Details: | Protein type:Receptor, misc.; Membrane protein, integral Chromosomal Location of Human Ortholog: 11q23 Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane Molecular Function:protein binding; transmembrane receptor activity; receptor signaling complex scaffold activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; SH3 domain binding; protein kinase binding Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; positive regulation of interleukin-4 production; regulation of apoptosis; G-protein coupled receptor protein signaling pathway; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; protein complex assembly; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell proliferation; positive regulation of T cell anergy; transmembrane receptor protein tyrosine kinase signaling pathway; response to nutrient Disease: Immunodeficiency 18 |
| NCBI Summary: | The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P07766 |
| NCBI GenInfo Identifier: | 1345708 |
| NCBI Gene ID: | 916 |
| NCBI Accession: | P07766.2 |
| UniProt Related Accession: | P07766 |
| Molecular Weight: | |
| NCBI Full Name: | T-cell surface glycoprotein CD3 epsilon chain |
| NCBI Synonym Full Names: | CD3e molecule |
| NCBI Official Symbol: | CD3E |
| NCBI Official Synonym Symbols: | T3E; TCRE; IMD18 |
| NCBI Protein Information: | T-cell surface glycoprotein CD3 epsilon chain |
| UniProt Protein Name: | T-cell surface glycoprotein CD3 epsilon chain |
| UniProt Synonym Protein Names: | T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e |
| UniProt Gene Name: | CD3E |
| UniProt Entry Name: | CD3E_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CD3E Antigen Antibody (CAB12415) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded mouse spleen using CD3E Antigen antibody (CAB12415) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse thymus using CD3E Antigen antibody (CAB12415) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of Jurkat cells using CD3E Antigen antibody (CAB12415) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of RAW264.7 cells using CD3E Antigen antibody (CAB12415) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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