Anti-CD34 Antibody (CAB0761)
- SKU:
- CAB0761
- Product type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CD34 Antibody |
| Antibody SKU: | CAB0761 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 32-290 of human CD34 (NP_001020280.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | A-431, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 32-290 of human CD34 (NP_001020280.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SLDN NGTA TPEL PTQG TFSN VSTN VSYQ ETTT PSTL GSTS LHPV SQHG NEAT TNIT ETTV KFTS TSVI TSVY GNTN SSVQ SQTS VIST VFTT PANV STPE TTLK PSLS PGNV SDLS TTST SLAT SPTK PYTS SSPI LSDI KAEI KCSG IREV KLTQ GICL EQNK TSSC AEFK KDRG EGLA RVLC GEEQ ADAD AGAQ VCSL LLAQ SEVR PQCL LLVL ANRT EISS KLQL MKKH QSDL KKLG ILDF TEQD VASH QSYS QKT |
| Gene ID: | 947 |
| Uniprot: | P28906 |
| Cellular Location: | Membrane, Single-pass type I membrane protein |
| Calculated MW: | 35kDa/40kDa |
| Observed MW: | 115-125kDa |
| Synonyms: | CD34, CD34 molecule, GIG3, MORT1 |
| Background: | The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CD34: Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. Belongs to the CD34 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell surface; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q32 Cellular Component: intercellular bridge; integral to plasma membrane; lysosome; perinuclear region of cytoplasm; apical plasma membrane; cytoplasm; basal plasma membrane; plasma membrane; external side of plasma membrane Molecular Function:sulfate binding; carbohydrate binding; transcription factor binding Biological Process: positive regulation of odontogenesis; regulation of immune response; negative regulation of nitric oxide biosynthetic process; glomerular filtration; cell motility involved in cell locomotion; negative regulation of blood coagulation; negative regulation of tumor necrosis factor production; signal transduction; positive regulation of interleukin-10 production; cell proliferation; cell-cell adhesion; positive regulation of angiogenesis; endothelial cell proliferation; regulation of blood pressure; tissue homeostasis; hemopoiesis; leukocyte migration |
| NCBI Summary: | The protein encoded by this gene may play a role in the attachment of stem cells to the bone marrow extracellular matrix or to stromal cells. This single-pass membrane protein is highly glycosylated and phosphorylated by protein kinase C. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011] |
| UniProt Code: | P28906 |
| NCBI GenInfo Identifier: | 3183511 |
| NCBI Gene ID: | 947 |
| NCBI Accession: | P28906.2 |
| UniProt Secondary Accession: | P28906,Q15970, Q15971, Q5JTA3, Q5JTA4, Q9UJB1, A8K664 |
| UniProt Related Accession: | P28906 |
| Molecular Weight: | 35,011 Da |
| NCBI Full Name: | Hematopoietic progenitor cell antigen CD34 |
| NCBI Synonym Full Names: | CD34 molecule |
| NCBI Official Symbol: | CD34 |
| NCBI Protein Information: | hematopoietic progenitor cell antigen CD34; CD34 antigen |
| UniProt Protein Name: | Hematopoietic progenitor cell antigen CD34 |
| UniProt Synonym Protein Names: | CD_antigen: CD34 |
| Protein Family: | Hematopoietic progenitor cell antigen |
| UniProt Gene Name: | CD34 |
| UniProt Entry Name: | CD34_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CD34 Antibody (CAB0761) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human placenta using CD34 Rabbit pAb (CAB0761) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of human placenta using CD34 antibody (CAB0761) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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