Anti-CD2AP Antibody (CAB7788)
- SKU:
- CAB7788
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-CD2AP Antibody |
| Antibody SKU: | CAB7788 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human CD2AP |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-431, Raji, MCF7, SW480, HL-60, Mouse kidney, Rat kidney |
| Immunogen: | A synthetic peptide of human CD2AP |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 23607 |
| Uniprot: | Q9Y5K6 |
| Cellular Location: | Cell projection, Cytoplasm, cytoskeleton, ruffle |
| Calculated MW: | 71kDa |
| Observed MW: | 90kDa |
| Synonyms: | CD2AP, CMS |
| Background: | This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease. |
| UniProt Protein Function: | CD2AP: an adapter protein between membrane proteins and the actin cytoskeleton. May play a role in receptor clustering and cytoskeletal polarity in the junction between T-cell and antigen-presenting cell. May anchor the podocyte slit diaphragm to the actin cytoskeleton in renal glomerolus. Required for cytokinesis. Links CD2 engagement to the WASp-evoked actin polymerization required for synapse formation and T cell activation. Attaches to membrane proteins via its three SH3 domains and attaches to and rearranges the cytoskeleton via its C-terminus. CD2AP?s interactions with the cytoskeleton result in receptor clustering and endocysosis as well as targeting proteins to degradative vesicles in the kidney. Mice carrying a heterozygous knockout of CD2AP show defects in kidney filtration, and mutations in the human gene are associated with focal segmental glomerulosclerosis (FSGS3). |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Adaptor/scaffold Chromosomal Location of Human Ortholog: 6p12 Cellular Component: ruffle; filamentous actin; cytoplasm; nucleolus; plasma membrane; intercellular junction; actin cytoskeleton Molecular Function:protein binding; structural constituent of cytoskeleton; SH3 domain binding Biological Process: mitosis; cell division; protein complex assembly; signal transduction; substrate-bound cell migration, cell extension; negative regulation of transforming growth factor-beta1 production Disease: Focal Segmental Glomerulosclerosis 3, Susceptibility To |
| NCBI Summary: | This gene encodes a scaffolding molecule that regulates the actin cytoskeleton. The protein directly interacts with filamentous actin and a variety of cell membrane proteins through multiple actin binding sites, SH3 domains, and a proline-rich region containing binding sites for SH3 domains. The cytoplasmic protein localizes to membrane ruffles, lipid rafts, and the leading edges of cells. It is implicated in dynamic actin remodeling and membrane trafficking that occurs during receptor endocytosis and cytokinesis. Haploinsufficiency of this gene is implicated in susceptibility to glomerular disease. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y5K6 |
| NCBI GenInfo Identifier: | 11321634 |
| NCBI Gene ID: | 23607 |
| NCBI Accession: | NP_036252.1 |
| UniProt Secondary Accession: | Q9Y5K6,Q5VYA3, Q9UG97, A6NL34, |
| UniProt Related Accession: | Q9Y5K6 |
| Molecular Weight: | 34.5kDa |
| NCBI Full Name: | CD2-associated protein |
| NCBI Synonym Full Names: | CD2-associated protein |
| NCBI Official Symbol: | CD2AP |
| NCBI Official Synonym Symbols: | CMS |
| NCBI Protein Information: | CD2-associated protein; adapter protein CMS; cas ligand with multiple SH3 domains; Cas ligand with multiple Src homology 3 (SH3) domains |
| UniProt Protein Name: | CD2-associated protein |
| UniProt Synonym Protein Names: | Adapter protein CMS; Cas ligand with multiple SH3 domains |
| Protein Family: | CD2-associated protein |
| UniProt Gene Name: | CD2AP |
| UniProt Entry Name: | CD2AP_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CD2AP antibody (CAB7788) at 1:500 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 60s. |