Anti-CD146 Antibody (MACO0606)
- SKU:
- MACO0606
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Mouse
- Isotype:
- IgG2a
- Application:
- ELISA
- Application:
- WB
- Application:
- IF
- Application:
- FC
- Antibody type:
- Monoclonal Antibody
- Conjugation:
- Unconjugated
Frequently bought together:
Description
| Product Name: | Anti-CD146 Antibody |
| Product Sku: | MACO0606 |
| Size: | 50ul |
| Host Species: | Mouse |
| Tested Applications: | ELISA, WB, IF, FC |
| Recommended Dilutions: | WB:1:500-1:2000, IF:1:50-1:200 |
| Species Reactivity: | Human |
| Immunogen: | Recombinant Human Cell surface glycoprotein MUC18 protein (50-646AA) |
| Form: | Liquid |
| Storage Buffer: | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
| Purification Method: | >95%, Protein G purified |
| Clonality: | Monoclonal |
| Isotype: | IgG2a |
| Conjugate: | Non-conjugated |
| Background: | Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. |
| Synonyms: | Cell surface glycoprotein MUC18 (Cell surface glycoprotein P1H12) (Melanoma cell adhesion molecule) (Melanoma-associated antigen A32) (Melanoma-associated antigen MUC18) (S-endo 1 endothelial-associated antigen) (CD antigen CD146), MCAM, MUC18 |
 | Western Blot Positive WB detected in: Hela whole cell lysate, 293 whole cell lysate All lanes: CD146 antibody at 1:500 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 72, 58 kDa Observed band size: 120 kDa |
 | Immunofluorescence staining of A375 cells with MACO0606 at 1:100, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L). |
 | Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: CD146 antibody at 1:1250 Secondary Goat polyclonal to Mouse IgG at 1/50000 dilution Predicted band size: 72, 58 kDa Observed band size: 120 kDa |
| UniProt Protein Function: | MCAM: Plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Its expression may allow melanoma cells to interact with cellular elements of the vascular system, thereby enhancing hematogeneous tumor spread. Could be an adhesion molecule active in neural crest cells during embryonic development. Acts as surface receptor that triggers tyrosine phosphorylation of FYN and PTK2/FAK1, and a transient increase in the intracellular calcium concentration. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell adhesion; Membrane protein, integral Chromosomal Location of Human Ortholog: 11q23.3 Cellular Component: extracellular space; focal adhesion; integral to membrane; plasma membrane; nucleus; external side of plasma membrane Biological Process: anatomical structure morphogenesis; glomerular filtration; angiogenesis; cell adhesion; positive regulation of cell migration |
| UniProt Code: | P43121 |
| NCBI GenInfo Identifier: | 85681878 |
| NCBI Gene ID: | 4162 |
| NCBI Accession: | P43121.2 |
| UniProt Secondary Accession: | P43121,O95812, Q59E86, Q6PHR3, Q6ZTR2, |
| UniProt Related Accession: | P43121 |
| Molecular Weight: | 646 |
| NCBI Full Name: | Cell surface glycoprotein MUC18 |
| NCBI Synonym Full Names: | melanoma cell adhesion molecule |
| NCBI Official Symbol: | MCAMÂ Â |
| NCBI Official Synonym Symbols: | CD146; MUC18Â Â |
| NCBI Protein Information: | cell surface glycoprotein MUC18; Gicerin; melanoma adhesion molecule; cell surface glycoprotein P1H12; melanoma-associated antigen A32; melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen |
| UniProt Protein Name: | Cell surface glycoprotein MUC18 |
| UniProt Synonym Protein Names: | Cell surface glycoprotein P1H12; Melanoma cell adhesion molecule; Melanoma-associated antigen A32; Melanoma-associated antigen MUC18; S-endo 1 endothelial-associated antigen; CD_antigen: CD146 |
| Protein Family: | Cell surface glycoprotein |
| UniProt Gene Name: | MCAMÂ Â |
| UniProt Entry Name: | MUC18_HUMAN |
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