Anti-CD133 Antibody (CAB0219)
- SKU:
- CAB0219
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CD133 Antibody |
| Antibody SKU: | CAB0219 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF IP |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-430 of human CD133 (NP_001139320.1). |
| Application: | WB IHC IF IP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HT-29, Mouse testis, Mouse kidney, Mouse pancreas, Rat lung, Rat kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-430 of human CD133 (NP_001139320.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | ANHQ VRTR IKRS RKLA DSNF KDLR TLLN ETPE QIKY ILAQ YNTT KDKA FTDL NSIN SVLG GGIL DRLR PNII PVLD EIKS MATA IKET KEAL ENMN STLK SLHQ QSTQ LSSS LTSV KTSL RSSL NDPL CLVH PSSE TCNS IRLS LSQL NSNP ELRQ LPPV DAEL DNVN NVLR TDLD GLVQ QGYQ SLND IPDR VQRQ TTTV VAGI KRVL NSIG SDID NVTQ RLPI QDIL SAFS VYVN NTES YIHR NLPT LEEY DSYW WLGG L |
| Gene ID: | 8842 |
| Uniprot: | O43490 |
| Cellular Location: | Apical cell membrane, Cell projection, Endoplasmic reticulum, Endoplasmic reticulum-Golgi intermediate compartment, Multi-pass membrane protein, cilium, microvillus membrane, photoreceptor outer segment |
| Calculated MW: | 92kDa/93kDa/94kDa/96kDa/97kDa |
| Observed MW: | 97kDa/125kDa |
| Synonyms: | PROM1, AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4 |
| Background: | This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | CD133: Binds cholesterol in cholesterol-containing plasma membrane microdomains. Proposed to play a role in apical plasma membrane organization of epithelial cells. During early retinal development acts as a key regulator of disk morphogenesis. Involved in regulation of MAPK and Akt signaling pathways. In neuroblastoma cells suppresses cell differentiation such as neurite outgrowth in a RET-dependent manner. Interacts with CDHR1 and with actin filaments. Isoform 1 is selectively expressed on CD34 hematopoietic stem and progenitor cells in adult and fetal bone marrow, fetal liver, cord blood and adult peripheral blood. Isoform 1 is not detected on other blood cells. Isoform 1 is also expressed in a number of non-lymphoid tissues including retina, pancreas, placenta, kidney, liver, lung, brain and heart. Found in saliva within small membrane particles. Isoform 2 is predominantly expressed in fetal liver, skeletal muscle, kidney, and heart as well as adult pancreas, kidney, liver, lung, and placenta. Isoform 2 is highly expressed in fetal liver, low in bone marrow, and barely detectable in peripheral blood. Isoform 2 is expressed on hematopoietic stem cells and in epidermal basal cells. Expressed in adult retina by rod and cone photoreceptor cells. Belongs to the prominin family. 7 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell surface; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 4p15.32 Cellular Component: stereocilium; extracellular space; cell surface; photoreceptor outer segment; microvillus membrane; endoplasmic reticulum; integral to plasma membrane; apical plasma membrane; plasma membrane; ER-Golgi intermediate compartment; vesicle; brush border Molecular Function:actinin binding; protein binding; cadherin binding Biological Process: photoreceptor cell maintenance; retina morphogenesis in camera-type eye Disease: Macular Dystrophy, Retinal, 2; Stargardt Disease 4; Retinitis Pigmentosa 41; Cone-rod Dystrophy 12 |
| NCBI Summary: | This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009] |
| UniProt Code: | O43490 |
| NCBI GenInfo Identifier: | 13124442 |
| NCBI Gene ID: | 8842 |
| NCBI Accession: | O43490.1 |
| UniProt Secondary Accession: | O43490,Q6SV49, Q6SV50, Q6SV51, Q6SV52, Q6SV53, Q96EN6 |
| UniProt Related Accession: | O43490 |
| Molecular Weight: | 97kDa |
| NCBI Full Name: | Prominin-1 |
| NCBI Synonym Full Names: | prominin 1 |
| NCBI Official Symbol: | PROM1 |
| NCBI Official Synonym Symbols: | RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061 |
| NCBI Protein Information: | prominin-1; hProminin; antigen AC133; prominin-like protein 1; hematopoietic stem cell antigen |
| UniProt Protein Name: | Prominin-1 |
| UniProt Synonym Protein Names: | Antigen AC133; Prominin-like protein 1; CD_antigen: CD133 |
| Protein Family: | Prominin |
| UniProt Gene Name: | PROM1 |
| UniProt Entry Name: | PROM1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CD133 antibody (CAB0219) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded human prostate using CD133 antibody (CAB0219) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human lung using CD133 antibody (CAB0219) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of C6 cells using CD133 antibody (CAB0219) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunoprecipitation analysis of 200ug extracts of HT-29 cells using 3ug CD133 antibody (CAB0219). Western blot was performed from the immunoprecipitate using CD133 antibody (CAB0219) at a dilition of 1:1000. |
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