Anti-CBFA2T3 Antibody (CAB14712)
- SKU:
- CAB14712
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CBFA2T3 Antibody |
| Antibody SKU: | CAB14712 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 305-390 of human CBFA2T3 (NP_005178.4). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human |
| Positive Samples: | K-562, U-251MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 305-390 of human CBFA2T3 (NP_005178.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DRDP LHPE HLSK RPCT LNPA QRYS PSNG PPQP TPPP HYRL EDIA MAHH FRDA YRHP DPRE LRER HRPL VVPG SRQE EVID HKLT ER |
| Gene ID: | 863 |
| Uniprot: | O75081 |
| Cellular Location: | Golgi apparatus membrane, Nucleus, nucleolus, nucleoplasm |
| Calculated MW: | 7kDa/62kDa/63kDa/71kDa |
| Observed MW: | 60kDa |
| Synonyms: | CBFA2T3, ETO2, MTG16, MTGR2, RUNX1T3, ZMYND4 |
| Background: | This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(16;21)(q24;q22) translocation is one of the less common karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. This gene is also a putative breast tumor suppressor. Alternative splicing results in transcript variants. |
| UniProt Protein Function: | Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes (PubMed:12559562, PubMed:15203199). Can repress the expression of MMP7 in a ZBTB33-dependent manner (PubMed:23251453). Reduces the protein levels and stability of the transcriptinal regulator HIF1A; interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway (PubMed:25974097). Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A (PubMed:23840896, PubMed:25974097). Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes. Plays a role in granulocyte differentiation (PubMed:15231665). |
| NCBI Summary: | This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(16;21)(q24;q22) translocation is one of the less common karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. This gene is also a putative breast tumor suppressor. Alternative splicing results in transcript variants. [provided by RefSeq, Nov 2010] |
| UniProt Code: | O75081 |
| NCBI GenInfo Identifier: | 28872803 |
| NCBI Gene ID: | 863 |
| NCBI Accession: | NP_005178.4 |
| UniProt Secondary Accession: | O75081,O60615, O60616, O60617, O75082, O75107, O75108 Q0P5Z6, Q6P5W6, D3DX78, |
| UniProt Related Accession: | O75081 |
| Molecular Weight: | 7,436 Da |
| NCBI Full Name: | protein CBFA2T3 isoform 1 |
| NCBI Synonym Full Names: | CBFA2/RUNX1 translocation partner 3 |
| NCBI Official Symbol: | CBFA2T3 |
| NCBI Official Synonym Symbols: | ETO2; MTG16; MTGR2; ZMYND4; RUNX1T3 |
| NCBI Protein Information: | protein CBFA2T3 |
| UniProt Protein Name: | Protein CBFA2T3 |
| UniProt Synonym Protein Names: | MTG8-related protein 2; Myeloid translocation gene on chromosome 16 protein; hMTG16; Zinc finger MYND domain-containing protein 4 |
| UniProt Gene Name: | CBFA2T3 |
 | Western blot analysis of extracts of various cell lines, using CBFA2T3 antibody (CAB14712) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
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