Anti-Caspase-12 Antibody (CAB0217)
- SKU:
- CAB0217
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-Caspase-12 Antibody |
| Antibody SKU: | CAB0217 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Caspase-12 (NP_001177945.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, Mouse lung, Mouse ovary, Mouse uterus, Rat lung |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Caspase-12 (NP_001177945.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MADE KPSN GVLV HMVK LLIK TFLD GIFD DLME NNVL NTDE IHLI GKCL KFVV SNAE NLVD DITE TAQI AGKI FREH LWNS KKQL SSDI SSDG EREA NMPG |
| Gene ID: | 100506742 |
| Uniprot: | Q6UXS9 |
| Cellular Location: | |
| Calculated MW: | 19kDa/29kDa/38kDa |
| Observed MW: | 42kDa |
| Synonyms: | CASP12, CASP-12, CASP12P1 |
| Background: |
| UniProt Protein Function: | CASP12: Has no protease activity. May reduce cytokine release in response to bacterial lipopolysaccharide during infections. Reduces activation of NF-kappa-B in response to TNF. Detected in heart, kidney, liver, lung, pancreas, small intestine, spleen, stomach, thymus and testis. Belongs to the peptidase C14A family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protease; EC 3.4.22.- Chromosomal Location of Human Ortholog: 11q22.3 Cellular Component: cytoplasm Molecular Function:cysteine-type endopeptidase activity Biological Process: regulation of apoptosis; unfolded protein response; regulation of inflammatory response; proteolysis |
| UniProt Code: | Q6UXS9 |
| NCBI GenInfo Identifier: | 395398427 |
| NCBI Gene ID: | |
| NCBI Accession: | Q6UXS9.2 |
| Molecular Weight: | |
| NCBI Full Name: | Inactive caspase-12 |
| UniProt Protein Name: | Inactive caspase-12 |
| UniProt Gene Name: | CASP12 |
| UniProt Entry Name: | CASPC_HUMAN |
 | Western blot analysis of extracts of various cell lines, using Caspase-12 Antibody (CAB0217) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human prostate using Caspase-12 antibody (CAB0217) at dilution of 1:100 (20x lens). |
 | Immunohistochemistry of paraffin-embedded human stomach using Caspase-12 antibody (CAB0217) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using Caspase-12 Polyclonal Antibody (CAB0217) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using Caspase-12 Polyclonal Antibody (CAB0217) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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