Anti-CASP8AP2 항체 (CAB15770)

Antibody Name:	Anti-CASP8AP2 Antibody
Antibody SKU:	CAB15770
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1733-1982 of human CASP8AP2 (NP_036247.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human
Positive Samples:	U-87MG, K-562

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1733-1982 of human CASP8AP2 (NP_036247.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TVGA FIDL TQDA SSEA KSEG NHPA LAVE DLGC GVIQ VDED NCKE EKAQ VANR PLKC IVEE TYID LTTE SPSS CEVK KDEL KSEP GSNC DNSE LPGT LHNS HKKR RNIS DLNH PHKK QRKE TDLT NKEK TKKP TQDS CENT EAHQ KKAS KKKA PPVT KDPS SLKA TPGI KDSS AALA TSTS LSAK NVIK KKGE IIIL WTRN DDRE ILLE CQKR GPSF KTFA YLAA KLDK NPNQ VSER FQQL MKLF EKSK CR
Gene ID:	9994
Uniprot:	Q9UKL3
Cellular Location:	Cytoplasm, Mitochondrion, Nucleus, PML body
Calculated MW:	222kDa
Observed MW:	215kDa

Synonyms:

CASP8AP2, CED-4, FLASH, RIP25

Background:

This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.

UniProt Protein Function:	CASP8AP2: Participates in TNF-alpha-induced blockade of glucocorticoid receptor (GR) transactivation at the nuclear receptor coactivator level, upstream and independently of NF- kappa-B. Suppresses both NCOA2- and NCOA3-induced enhancement of GR transactivation. Involved in TNF-alpha-induced activation of NF-kappa-B via a TRAF2-dependent pathway. Acts as a downstream mediator for CASP8-induced activation of NF-kappa-B. Required for the activation of CASP8 in FAS-mediated apoptosis. Required for histone gene transcription and progression through S phase.
UniProt Protein Details:	Protein type:Apoptosis; Nuclear receptor co-regulator Chromosomal Location of Human Ortholog: 6q15 Cellular Component: PML body; mitochondrion; cytoplasm; nucleus Molecular Function:protein binding; DNA binding; SUMO polymer binding; death receptor binding; apoptotic protease activator activity; caspase activator activity; transcription corepressor activity Biological Process: caspase activation; regulation of transcription, DNA-dependent; induction of apoptosis via death domain receptors; transcription, DNA-dependent; signal transduction; cell cycle
NCBI Summary:	This protein is highly similar to FLASH, a mouse apoptotic protein identified by its interaction with the death-effector domain (DED) of caspase 8. Studies of FLASH protein suggested that this protein may be a component of the death-inducing signaling complex that includes Fas receptor, Fas-binding adapter FADD, and caspase 8, and plays a regulatory role in Fas-mediated apoptosis. Alternative splicing results in multiple transcript variants encoding the same protein.[provided by RefSeq, Nov 2008]
UniProt Code:	Q9UKL3
NCBI GenInfo Identifier:	5533376
NCBI Gene ID:	9994
NCBI Accession:	AF165161
UniProt Secondary Accession:	Q9UKL3,Q6PH76, Q7LCQ7, Q86YD9, Q9NUQ4, Q9NZV9, Q9P2N1 Q9Y563, A2RUB7, E1P553,
UniProt Related Accession:	Q9UKL3
Molecular Weight:	222,658 Da
NCBI Full Name:	Homo sapiens FLASH mRNA, partial cds
NCBI Synonym Full Names:	caspase 8 associated protein 2
NCBI Official Symbol:	CASP8AP2
NCBI Official Synonym Symbols:	CED-4; FLASH; RIP25
NCBI Protein Information:	CASP8-associated protein 2
UniProt Protein Name:	CASP8-associated protein 2
UniProt Synonym Protein Names:	FLICE-associated huge protein
UniProt Gene Name:	CASP8AP2
UniProt Entry Name:	C8AP2_HUMAN

Western blot analysis of extracts of various cell lines, using CASP8AP2 antibody (CAB15770) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)