Anti-CAMK2G Antibody (CAB3231)
- SKU:
- CAB3231
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CAMK2G Antibody |
| Antibody SKU: | CAB3231 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-410 of human CAMK2G (NP_751909.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SH-SY5Y, HeLa, HL-60, 22Rv1, Mouse heart, Rat heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 300-410 of human CAMK2G (NP_751909.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LKGA ILTT MLVS RNFS VGRQ SSAP ASPA ASAA GLAG QAAK SLLN KKSD GGVK KRKS SSSV HLME PQTT VVHN ATDG IKGS TESC NTTT EDED LKVR KQEI IKIT EQLI EAI |
| Gene ID: | 818 |
| Uniprot: | Q13555 |
| Cellular Location: | Cytoplasmic side, Peripheral membrane protein, Sarcoplasmic reticulum membrane |
| Calculated MW: | 55kDa/58kDa/59kDa/60kDa/61kDa/62kDa/65kDa |
| Observed MW: | 75kDa/80kDa |
| Synonyms: | CAMK2G, CAMK, CAMK-II, CAMKG |
| Background: | The product of this gene is one of the four subunits of an enzyme which belongs to the serine/threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. Calcium signaling is crucial for several aspects of plasticity at glutamatergic synapses. In mammalian cells the enzyme is composed of four different chains: alpha, beta, gamma, and delta. The product of this gene is a gamma chain. Many alternatively spliced transcripts encoding different isoforms have been described but the full-length nature of all the variants has not been determined. |
| UniProt Protein Function: | CAMK2G: a protein kinase of the CAMK2 family. A prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. Member of the NMDAR signaling complex in excitatory synapses that may regulate NMDAR-dependent potentiation of the AMPAR and synaptic plasticity. The holoenzyme is composed of four different chains: alpha, beta, gamma, and delta. The different chains assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. May interact with BAALC, MPDZ, SYN1 and synGAP. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.17; Protein kinase, CAMK; CAMK group; CAMK2 family Chromosomal Location of Human Ortholog: 10q22 Cellular Component: nucleoplasm; sarcoplasmic reticulum membrane; membrane; plasma membrane; cytosol Molecular Function:calmodulin binding; protein binding; protein homodimerization activity; calcium-dependent protein serine/threonine phosphatase activity; calmodulin-dependent protein kinase activity; ATP binding Biological Process: nervous system development; synaptic transmission; dephosphorylation; regulation of skeletal muscle adaptation; insulin secretion; calcium ion transport; protein amino acid autophosphorylation; cytokine and chemokine mediated signaling pathway; regulation of calcium ion transport; cell differentiation; protein oligomerization; G1/S transition of mitotic cell cycle |
| UniProt Code: | Q13555 |
| NCBI GenInfo Identifier: | 62512173 |
| NCBI Gene ID: | |
| NCBI Accession: | Q13555.3 |
| UniProt Secondary Accession: | Q13555,Q13554, |
| Molecular Weight: | ~ 50~80kDa |
| NCBI Full Name: | Calcium/calmodulin-dependent protein kinase type II subunit gamma |
| UniProt Protein Name: | Calcium/calmodulin-dependent protein kinase type II subunit gamma |
| Protein Family: | Calcium/calmodulin-dependent protein kinase |
| UniProt Gene Name: | CAMK2G |
| UniProt Entry Name: | KCC2G_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CAMK2G antibody (CAB3231) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human stomach using CAMK2G antibody (CAB3231) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using CAMK2G antibody (CAB3231) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse heart using CAMK2G antibody (CAB3231) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using CAMK2G Polyclonal Antibody (CAB3231) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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