Anti-CALD1 Antibody (CAB5366)
- SKU:
- CAB5366
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-CALD1 Antibody |
| Antibody SKU: | CAB5366 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human CALD1 (NP_004333.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | HeLa, 22Rv1, U-251MG, U-87MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human CALD1 (NP_004333.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDDF ERRR ELRR QKRE EMRL EAER IAYQ RNDD DEEE AARE RRRR ARQE RLRQ KQEE ESLG QVTD QVEV NAQN SVPD EEAK TTTT NTQV EGDD EAAF LERL ARRE ERRQ KRLQ EALE RQKE FDPT ITDA SLSL PSRR MQND TAEN ETTE KEEK SESR QERY EIEE TETV TKSY QKND WRDA EENK KEDK EKEE EEEE KPKR GSIG ENQI KDEK IKKD KEPK EEVK SFMD RKKG FTEV KSQN |
| Gene ID: | 800 |
| Uniprot: | Q05682 |
| Cellular Location: | Cytoplasm, cytoskeleton, myofibril |
| Calculated MW: | 61kDa/62kDa/64kDa/65kDa/93kDa |
| Observed MW: | 85kDa |
| Synonyms: | CALD1, CDM, H-CAD, HCAD, L-CAD, LCAD, NAG22, caldesmon |
| Background: | This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. |
| UniProt Protein Function: | Caldesmon: a cytoskeletal protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells. May act as a bridge between myosin and actin filaments. Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also plays an essential role during cellular mitosis and receptor capping. Five alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Actin-binding Chromosomal Location of Human Ortholog: 7q33 Cellular Component: actin cap; actin cytoskeleton; cytoskeleton; cytosol; myofibril; plasma membrane Molecular Function:actin binding; calmodulin binding; myosin binding; protein binding; tropomyosin binding Biological Process: cell motility; muscle contraction |
| NCBI Summary: | This gene encodes a calmodulin- and actin-binding protein that plays an essential role in the regulation of smooth muscle and nonmuscle contraction. The conserved domain of this protein possesses the binding activities to Ca(2+)-calmodulin, actin, tropomyosin, myosin, and phospholipids. This protein is a potent inhibitor of the actin-tropomyosin activated myosin MgATPase, and serves as a mediating factor for Ca(2+)-dependent inhibition of smooth muscle contraction. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q05682 |
| NCBI GenInfo Identifier: | 338817891 |
| NCBI Gene ID: | 800 |
| NCBI Accession: | Q05682.3 |
| UniProt Secondary Accession: | Q05682,Q13978, Q13979, Q14741, Q14742, Q9UD91, A8K0X1 |
| UniProt Related Accession: | Q05682 |
| Molecular Weight: | 65,620 Da |
| NCBI Full Name: | Caldesmon |
| NCBI Synonym Full Names: | caldesmon 1 |
| NCBI Official Symbol: | CALD1 |
| NCBI Official Synonym Symbols: | CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22 |
| NCBI Protein Information: | caldesmon |
| UniProt Protein Name: | Caldesmon |
| Protein Family: | Caldesmon |
| UniProt Gene Name: | CALD1 |
| UniProt Entry Name: | CALD1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using CALD1 antibody (CAB5366) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded human liver damage using CALD1 antibody (CAB5366) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using CALD1 antibody (CAB5366) at dilution of 1:100 (40x lens). |
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