Anti-CA2 Antibody (CAB18034)[KO Validated]

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SKU:
CAB18034
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-CA2 Antibody [KO Validated]
Antibody SKU:CAB18034
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CA2 (NP_000058.1).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:10 - 1:100
Reactivity:Human, Mouse
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human CA2 (NP_000058.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSHH WGYG KHNG PEHW HKDF PIAK GERQ SPVD IDTH TAKY DPSL KPLS VSYD QATS LRIL NNGH AFNV EFDD SQDK AVLK GGPL DGTY RLIQ FHFH WGSL DGQG SEHT VDKK KYAA ELHL VHWN TKYG DFGK AVQQ PDGL AVLG IFLK VGSA KPGL QKVV DVLD SIKT KGKS ADFT NFDP RGLL PESL DYWT YPGS LTTP PLLE CVTW IVLK EPIS VSSE QVLK FRKL NFNG EGEP EELM VDNW RPAQ PL
Gene ID:760
Uniprot:P00918
Cellular Location:Cell membrane, Cytoplasm
Calculated MW:29kDa
Observed MW:28kDa
Anti-CA2 Antibody [KO Validated] (CAB18034)Western blot analysis of extracts from normal (control) and CA2 knockout (KO) 293T cells, using CA2 antibody (CAB18034) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
Synonyms:CA2, CA-II, CAC, CAII, Car2, HEL-76, HEL-S-282
Background:The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:CA2: Essential for bone resorption and osteoclast differentiation. Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Interacts with SLC4A4. Interaction with SLC4A7 regulates SLC4A7 transporter activity. Activated by X-ray, histamine, L-adrenaline, L- and D-phenylalanine, L- and D-histidine, L-His-OMe and beta-Ala- His (carnosine). Competitively inhibited by saccharin, thioxolone, coumarins, 667-coumate, celecoxib (Celebrex), valdecoxib (Bextra), SC-125, SC-560, diclofenac, acetate, azide, bromide, sulfonamide derivatives such as acetazolamide (AZA), methazolamide (MZA), ethoxzolamide (EZA), dichlorophenamide (DCP), brinzolamide, dansylamide, thiabendazole-5-sulfonamide, trifluoromethane sulfonamide and N-hydroxysulfamide, fructose-based sugar sulfamate RWJ-37497, and Foscarnet (phosphonoformate trisodium salt). Repressed strongly by hydrogen sulfide(HS) and weakly by nitrate (NO(3)). Esterase activity weakly reduced by cyanamide. N- hydroxyurea interfers with zinc binding and inhibit activity. Belongs to the alpha-carbonic anhydrase family.
UniProt Protein Details:

Protein type:Lyase; EC 4.2.1.1; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 8q22

Cellular Component: extracellular space; microvillus; apical part of cell; axon; basolateral plasma membrane; cytoplasm; plasma membrane; cytosol; myelin sheath

Molecular Function:protein binding; carbonate dehydratase activity; zinc ion binding

Biological Process: secretion; positive regulation of osteoclast differentiation; positive regulation of synaptic transmission, GABAergic; positive regulation of cellular pH reduction; one-carbon compound metabolic process; odontogenesis of dentine-containing teeth; response to zinc ion; bicarbonate transport; response to estrogen stimulus; regulation of intracellular pH; positive regulation of bone resorption; morphogenesis of an epithelium; kidney development; response to pH

Disease: Osteopetrosis, Autosomal Recessive 3

NCBI Summary:The protein encoded by this gene is one of several isozymes of carbonic anhydrase, which catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2014]
UniProt Code:P00918
NCBI GenInfo Identifier:115456
NCBI Gene ID:760
NCBI Accession:P00918.2
UniProt Secondary Accession:P00918,Q6FI12, Q96ET9, B2R7G8,
UniProt Related Accession:P00918
Molecular Weight:260
NCBI Full Name:Carbonic anhydrase 2
NCBI Synonym Full Names:carbonic anhydrase II
NCBI Official Symbol:CA2  
NCBI Official Synonym Symbols:CAC; CAII; Car2; CA-II; HEL-76  
NCBI Protein Information:carbonic anhydrase 2; carbonic anhydrase B; carbonic anhydrase C; carbonic dehydratase; carbonate dehydratase II; epididymis luminal protein 76
UniProt Protein Name:Carbonic anhydrase 2
UniProt Synonym Protein Names:Carbonate dehydratase II; Carbonic anhydrase C; CAC; Carbonic anhydrase II; CA-II
Protein Family:Beta-carotene hydroxylase
UniProt Gene Name:CA2  
UniProt Entry Name:CAH2_HUMAN

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