Anti-c-Myc Antibody [KO Validated] (CAB19032)

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SKU:
CAB19032
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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Antibody Name:Anti-c-Myc Antibody [KO Validated]
Antibody SKU:CAB19032
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human c-Myc
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HCT116, Jurkat, MCF7, Mouse ovary
Immunogen:A synthesized peptide derived from human c-Myc
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4609
Uniprot:P01106
Cellular Location:Nucleus, nucleolus, nucleoplasm
Calculated MW:57kDa
Observed MW:50-60kDa
Anti-c-Myc Antibody [KO Validated] (CAB19032)Western blot analysis of extracts from normal (control) and c-Myc knockout (KO) HCT116 cells, using c-Myc antibody (CAB19032) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-c-Myc Antibody [KO Validated] (CAB19032)Western blot analysis of extracts of various cell lines, using c-Myc antibody (CAB19032) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Anti-c-Myc Antibody [KO Validated] (CAB19032)Western blot analysis of extracts of Mouse ovary, using c-Myc antibody (CAB19032) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 3min.
Anti-c-Myc Antibody [KO Validated] (CAB19032)Immunofluorescence analysis of HeLa cells using c-Myc Rabbit mAb (CAB19032) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Synonyms:MRTL, MYCC, bHLHe39, c-Myc, MYC
Background:The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]
UniProt Protein Function:Myc: a proto-oncogenic transcription factor that plays a role in cell proliferation, apoptosis and in the development of human tumors. Seems to activate the transcription of growth-related genes.
UniProt Protein Details:

Protein type:DNA-binding; Nucleolus; Oncoprotein; Transcription factor

Chromosomal Location of Human Ortholog: 8q24.21

Cellular Component: nucleoplasm; protein complex; nucleolus; nucleus; cytosol

Molecular Function:protein dimerization activity; protein binding; DNA binding; protein complex binding; transcription factor binding; transcription factor activity

Biological Process: oxygen transport; cellular iron ion homeostasis; positive regulation of transcription, DNA-dependent; positive regulation of caspase activity; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; chromosome organization and biogenesis; positive regulation of fibroblast proliferation; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; response to gamma radiation; cell cycle arrest; response to drug; transcription initiation from RNA polymerase II promoter; Notch signaling pathway; regulation of telomere maintenance; transcription, DNA-dependent; MAPKKK cascade; negative regulation of cell division; negative regulation of stress-activated MAPK cascade; negative regulation of monocyte differentiation; chromatin remodeling; ureteric bud branching; regulation of gene expression; negative regulation of fibroblast proliferation; energy reserve metabolic process; gene expression; positive regulation of transcription from RNA polymerase II promoter; response to DNA damage stimulus; positive regulation of epithelial cell proliferation; negative regulation of apoptosis

Disease: Burkitt Lymphoma

NCBI Summary:The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P01106
NCBI GenInfo Identifier:127619
NCBI Gene ID:4609
NCBI Accession:P01106.1
UniProt Secondary Accession:P01106,P01107, Q14026, A8WFE7,
UniProt Related Accession:P01106
Molecular Weight:439
NCBI Full Name:Myc proto-oncogene protein
NCBI Synonym Full Names:v-myc avian myelocytomatosis viral oncogene homolog
NCBI Official Symbol:MYC  
NCBI Official Synonym Symbols:MRTL; MYCC; c-Myc; bHLHe39  
NCBI Protein Information:myc proto-oncogene protein; proto-oncogene c-Myc; transcription factor p64; class E basic helix-loop-helix protein 39; avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog; myc-related translation/localization regulatory factor
UniProt Protein Name:Myc proto-oncogene protein
UniProt Synonym Protein Names:Class E basic helix-loop-helix protein 39; bHLHe39; Proto-oncogene c-Myc; Transcription factor p64
Protein Family:Myc protein
UniProt Gene Name:MYC  
UniProt Entry Name:MYC_HUMAN

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