Anti-BUB3 Antibody (CAB6536)
- SKU:
- CAB6536
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-BUB3 Antibody |
| Antibody SKU: | CAB6536 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human BUB3 (NP_004716.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, HL-60, HeLa, Mouse spleen, Mouse ovary, Mouse brain, Mouse lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human BUB3 (NP_004716.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MTGS NEFK LNQP PEDG ISSV KFSP NTSQ FLLV SSWD TSVR LYDV PANS MRLK YQHT GAVL DCAF YDPT HAWS GGLD HQLK MHDL NTDQ ENLV GTHD APIR CVEY CPEV NVMV TGSW DQTV KLWD PRTP CNAG TFSQ PEKV YTLS VSGD RLIV GTAG RRVL VWDL RNMG YVQQ RRES SLKY QTRC IRAF PNKQ GYVL SSIE GRVA VEYL DPSP EVQK KKYA FKCH RLKE NNIE QIYP VNAI SFHN IHNT FATG GSDG FVNI WDPF NKKR LCQF HRYP TSIA SLAF SNDG TTLA IASS YMYE MDDT EHPE DGIF IRQV TDAE TKPK SPCT |
| Gene ID: | 9184 |
| Uniprot: | O43684 |
| Cellular Location: | Chromosome, Nucleus, centromere, kinetochore |
| Calculated MW: | 36kDa/37kDa |
| Observed MW: | 40kDa |
| Synonyms: | BUB3, BUB3L, hBUB3 |
| Background: | This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. |
| UniProt Protein Function: | BUB3: Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Interacts with BUB1 and BUBR1. The BUB1/BUB3 complex interacts with MAD1L1. Belongs to the WD repeat BUB3 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Cell cycle regulation Chromosomal Location of Human Ortholog: 10q26 Cellular Component: kinetochore; nucleoplasm; cytosol Molecular Function:protein binding Biological Process: negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; mitotic sister chromatid segregation; meiotic cell cycle; cell division; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle spindle assembly checkpoint; mitotic cell cycle; attachment of spindle microtubules to kinetochore |
| NCBI Summary: | This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O43684 |
| NCBI GenInfo Identifier: | 7387554 |
| NCBI Gene ID: | 9184 |
| NCBI Accession: | O43684.1 |
| UniProt Secondary Accession: | O43684,O43685, A6NJ42, B2R6E7, D3DRE9, |
| UniProt Related Accession: | O43684 |
| Molecular Weight: | 328 |
| NCBI Full Name: | Mitotic checkpoint protein BUB3 |
| NCBI Synonym Full Names: | BUB3 mitotic checkpoint protein |
| NCBI Official Symbol: | BUB3 |
| NCBI Official Synonym Symbols: | BUB3L; hBUB3 |
| NCBI Protein Information: | mitotic checkpoint protein BUB3; mitotic checkpoint component; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog; BUB3 budding uninhibited by benzimidazoles 3 homolog |
| UniProt Protein Name: | Mitotic checkpoint protein BUB3 |
| Protein Family: | BUB3-interacting and GLEBS motif-containing protein |
| UniProt Gene Name: | BUB3 |
| UniProt Entry Name: | BUB3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BUB3 antibody (CAB6536) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunofluorescence analysis of U2OS cells using BUB3 antibody (CAB6536). |
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