Anti-BTAF1 Antibody (CAB5811)
- SKU:
- CAB5811
- Product type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-BTAF1 Antibody |
| Antibody SKU: | CAB5811 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IP ChIP |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1600-1849 of human BTAF1 (NP_003963.1). |
| Application: | WB IHC IP ChIP |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:20 - 1:50 ChIP 1:20 - 1:100 |
| Reactivity: | Human, Rat |
| Positive Samples: | HepG2, HeLa, MCF7, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1600-1849 of human BTAF1 (NP_003963.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LAVQ NSSL HDIQ HAPK LSAL KQLL LDCG LGNG STSE SGTE SVVA QHRI LIFC QLKS MLDI VEHD LLKP HLPS VTYL RLDG SIPP GQRH SIVS RFNN DPSI DVLL LTTH VGGL GLNL TGAD TVVF VEHD WNPM RDLQ AMDR AHRI GQKR VVNV YRLI TRGT LEEK IMGL QKFK MNIA NTVI SQEN SSLQ SMGT DQLL DLFT LDKD GKAE KADT STSG KASM KSIL ENLS DLWD QEQY DSEY SLEN FMHS LK |
| Gene ID: | 9044 |
| Uniprot: | O14981 |
| Cellular Location: | Nucleus |
| Calculated MW: | 76kDa/206kDa |
| Observed MW: | 250kDa |
| Synonyms: | BTAF1, MOT1, TAF(II)170, TAF172, TAFII170 |
| Background: | This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters. |
| UniProt Protein Function: | TAF172: Regulates transcription in association with TATA binding protein (TBP). Removes TBP from the TATA box in an ATP-dependent manner. Associates with TBP to form B-TFIID. Binds DRAP1. Belongs to the SNF2/RAD54 helicase family. |
| UniProt Protein Details: | Protein type:Transcription initiation complex; Helicase; EC 3.6.4.-; EC 3.6.1.- Chromosomal Location of Human Ortholog: 10q22-q23 Cellular Component: nucleus Molecular Function:DNA binding; transcription factor activity; helicase activity; ATP binding Biological Process: metabolic process; negative regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene encodes a TAF (TATA box-binding protein-associated factor), which associates with TBP (TATA box-binding protein) to form the B-TFIID complex that is required for transcription initiation of genes by RNA polymerase II. This TAF has DNA-dependent ATPase activity, which drives the dissociation of TBP from DNA, freeing the TBP to associate with other TATA boxes or TATA-less promoters. [provided by RefSeq, Sep 2011] |
| UniProt Code: | O14981 |
| NCBI GenInfo Identifier: | 12643543 |
| NCBI Gene ID: | 9044 |
| NCBI Accession: | O14981.2 |
| UniProt Secondary Accession: | O14981,O43578, B4E0W6, |
| UniProt Related Accession: | O14981 |
| Molecular Weight: | 1849 |
| NCBI Full Name: | TATA-binding protein-associated factor 172 |
| NCBI Synonym Full Names: | BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa |
| NCBI Official Symbol: | BTAF1 |
| NCBI Official Synonym Symbols: | MOT1; TAF172; TAFII170; TAF(II)170 |
| NCBI Protein Information: | TATA-binding protein-associated factor 172; Mot1 homolog; TBP-associated factor 172; ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit; BTAF1 RNA polymerase II, B-TFIID transcription factor-associated, 170kDa (Mot1 homolog, S. cerevisiae) |
| UniProt Protein Name: | TATA-binding protein-associated factor 172 |
| UniProt Synonym Protein Names: | ATP-dependent helicase BTAF1; B-TFIID transcription factor-associated 170 kDa subunit; TAF(II)170; TBP-associated factor 172; TAF-172 |
| Protein Family: | TATA-binding protein-associated factor |
| UniProt Gene Name: | BTAF1 |
| UniProt Entry Name: | BTAF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BTAF1 antibody (CAB5811) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
 | Immunoprecipitation analysis of 150ug extracts of HeLa cells using 3ug BTAF1 antibody (CAB5811). Western blot was performed from the immunoprecipitate using BTAF1 antibody (CAB5811) at a dilition of 1:500. |
 | Chromatin immunoprecipitation analysis of extracts of HeLa cells, using BTAF1 antibody (CAB5811) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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