Anti-BRMS1 Antibody (CAB14865)
- SKU:
- CAB14865
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-BRMS1 Antibody |
| Antibody SKU: | CAB14865 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-210 of human BRMS1 (NP_056214.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse kidney, Mouse testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-210 of human BRMS1 (NP_056214.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | LEEV GAER APEY TEPL GGLQ RSLK IRIQ VAGI YKGF CLDV IRNK YECE LQGA KQHL ESEK LLLY DTLQ GELQ ERIQ RLEE DRQS LDLS SEWW DDKL HARG SSRS WDSL PPSK RKKA PLVS G |
| Gene ID: | 25855 |
| Uniprot: | Q9HCU9 |
| Cellular Location: | Cytoplasm, Nucleus |
| Calculated MW: | 28kDa |
| Observed MW: | 28kDa |
| Synonyms: | BRMS1 |
| Background: | This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. |
| UniProt Protein Function: | BRMS1: Transcriptional repressor. Down-regulates transcription activation by NF-kappa-B by promoting the deacetylation of RELA at 'Lys-310'. Promotes HDAC1 binding to promoter regions. Down- regulates expression of anti-apoptotic genes that are controlled by NF-kappa-B. Promotes apoptosis in cells that have inadequate adherence to a substrate, a process called anoikis, and may thereby inhibit metastasis. May be a mediator of metastasis suppression in breast carcinoma. Belongs to the BRMS1 family. |
| UniProt Protein Details: | Protein type:Tumor suppressor Chromosomal Location of Human Ortholog: 11q13.2 Cellular Component: cytoplasm; nucleoplasm; nucleus Molecular Function:histone deacetylase activity; histone deacetylase binding; NF-kappaB binding; protein binding Biological Process: histone deacetylation; inhibition of NF-kappaB transcription factor; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent |
| NCBI Summary: | This gene reduces the metastatic potential, but not the tumorogenicity, of human breast cancer and melanoma cell lines. The protein encoded by this gene localizes primarily to the nucleus and is a component of the mSin3a family of histone deacetylase complexes (HDAC). The protein contains two coiled-coil motifs and several imperfect leucine zipper motifs. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9HCU9 |
| NCBI GenInfo Identifier: | 18202959 |
| NCBI Gene ID: | 25855 |
| NCBI Accession: | Q9HCU9.1 |
| UniProt Secondary Accession: | Q9HCU9,Q6IAI2, |
| UniProt Related Accession: | Q9HCU9 |
| Molecular Weight: | 28,461 Da |
| NCBI Full Name: | Breast cancer metastasis-suppressor 1 |
| NCBI Synonym Full Names: | breast cancer metastasis suppressor 1 |
| NCBI Official Symbol: | BRMS1 |
| NCBI Protein Information: | breast cancer metastasis-suppressor 1 |
| UniProt Protein Name: | Breast cancer metastasis-suppressor 1 |
| Protein Family: | Breast cancer metastasis-suppressor |
| UniProt Gene Name: | BRMS1 |
| UniProt Entry Name: | BRMS1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BRMS1 antibody (CAB14865) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry of paraffin-embedded rat liver using BRMS1 antibody (CAB14865) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon using BRMS1 antibody (CAB14865) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using BRMS1 antibody (CAB14865) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of H9C2 cells using BRMS1 Rabbit pAb (CAB14865) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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