Anti-BRCA2 Antibody (CAB2435)
- SKU:
- CAB2435
- Product type:
- Antibody
- Reactivity:
- Human
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-BRCA2 Antibody |
| Antibody SKU: | CAB2435 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BRCA2 (NP_000050.2). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Rat |
| Positive Samples: | 293T |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BRCA2 (NP_000050.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MPIG SKER PTFF EIFK TRCN KADL GPIS LNWF EELS SEAP PYNS EPAE ESEH KNNN YEPN LFKT PQRK PSYN QLAS TPII FKEQ GLTL PLYQ SPVK ELDK FKLD LGRN VPNS RHKS LRTV KTKM DQAD DVSC PLLN SCLS ESPV VLQC THVT PQRD KSVV CGSL FHTP KFVK GRQT PKHI SESL GAEV DPDM SWSS SLAT PPTL SSTV LIVR NEEA SETV FPHD TTAN VKSY FSNH DESL KKND RFIA SVTD SENT NQRE AASH GFGK TSGN SFKV NSCK DHIG KSMP NVLE DEVY ETVV |
| Gene ID: | 675 |
| Uniprot: | P51587 |
| Cellular Location: | Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center |
| Calculated MW: | 384kDa |
| Observed MW: | 384kDa |
| Synonyms: | BRCA2, BRCC2, BROVCA2, FACD, FAD, FAD1, FANCD, FANCD1, GLM3, PNCA2, XRCC11 |
| Background: | Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. |
| UniProt Protein Function: | BRCA2: involved in double-strand break repair and/or homologous recombination. May participate in S phase checkpoint activation. Interacts with RAD51 and DSS1. Interacts with ubiquitinated FANCD2. Interacts with PALB2, enables the recombinational repair and checkpoint functions. Interacts with WDR16. Defects in BRCA2 are a cause of genetic susceptibility to breast cancer and may underlie susceptibility to uveal melanoma. |
| UniProt Protein Details: | Protein type:Tumor suppressor; Nuclear receptor co-regulator; DNA repair, damage Chromosomal Location of Human Ortholog: 13q12.3 Cellular Component: nucleoplasm; centrosome; protein complex; cytoplasm; BRCA2-MAGE-D1 complex; nucleus; secretory granule Molecular Function:gamma-tubulin binding; protein binding; H4 histone acetyltransferase activity; histone acetyltransferase activity; protease binding; H3 histone acetyltransferase activity; single-stranded DNA binding Biological Process: positive regulation of transcription, DNA-dependent; cytokinesis; cell aging; positive regulation of mitotic cell cycle; DNA repair; regulation of cytokinesis; oocyte maturation; inner cell mass cell proliferation; negative regulation of mammary gland epithelial cell proliferation; response to UV-C; double-strand break repair via homologous recombination; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; male meiosis I; nucleotide-excision repair; double-strand break repair; response to gamma radiation; hemopoiesis; spermatogenesis; replication fork protection; brain development; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; centrosome duplication; female gonad development; response to X-ray Disease: Pancreatic Cancer, Susceptibility To, 2; Breast-ovarian Cancer, Familial, Susceptibility To, 2; Prostate Cancer; Breast Cancer; Medulloblastoma; Glioma Susceptibility 3; Tracheoesophageal Fistula With Or Without Esophageal Atresia; Fanconi Anemia, Complementation Group D1; Wilms Tumor 1 |
| NCBI Summary: | Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele. [provided by RefSeq, Dec 2008] |
| UniProt Code: | P51587 |
| NCBI GenInfo Identifier: | 14424438 |
| NCBI Gene ID: | 675 |
| NCBI Accession: | P51587.2 |
| UniProt Secondary Accession: | P51587,O00183, O15008, Q13879, Q5TBJ7, |
| UniProt Related Accession: | P51587 |
| Molecular Weight: | 3418 |
| NCBI Full Name: | Breast cancer type 2 susceptibility protein |
| NCBI Synonym Full Names: | breast cancer 2, early onset |
| NCBI Official Symbol: | BRCA2 |
| NCBI Official Synonym Symbols: | FAD; FACD; FAD1; GLM3; BRCC2; FANCD; PNCA2; FANCD1; XRCC11; BROVCA2 |
| NCBI Protein Information: | breast cancer type 2 susceptibility protein; Fanconi anemia group D1 protein; breast cancer 2 tumor suppressor; BRCA1/BRCA2-containing complex, subunit 2; breast and ovarian cancer susceptibility gene, early onset |
| UniProt Protein Name: | Breast cancer type 2 susceptibility protein |
| UniProt Synonym Protein Names: | Fanconi anemia group D1 protein |
| Protein Family: | BRCA2 and CDKN1A-interacting protein |
| UniProt Gene Name: | BRCA2 |
| UniProt Entry Name: | BRCA2_HUMAN |
 | Western blot analysis of extracts of 293T cells, using BRCA2 antibody (CAB2435) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunofluorescence analysis of C6 cells using BRCA2 antibody (CAB2435) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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