Anti-BNIP3L 항체 (CAB6283)

Antibody Name:	Anti-BNIP3L Antibody
Antibody SKU:	CAB6283
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human BNIP3L (NP_004322.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, A-549, MCF7, Raji, Mouse spleen, Mouse thymus, Mouse brain, Mouse kidney

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-187 of human BNIP3L (NP_004322.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MSSH LVEP PPPL HNNN NNCE ENEQ SLPP PAGL NSSW VELP MNSS NGND NGNG KNGG LEHV PSSS SIHN GDME KILL DAQH ESGQ SSSR GSSH CDSP SPQE DGQI MFDV EMHT SRDH SSQS EEEV VEGE KEVE ALKK SADW VSDW SSRP ENIP PKEF HFRH PKRS VSLS MRKS GAMK KGGI FSAE FLK
Gene ID:	665
Uniprot:	O60238
Cellular Location:	Endoplasmic reticulum, Membrane, Mitochondrion outer membrane, Nucleus envelope, Single-pass membrane protein
Calculated MW:	19kDa/23kDa
Observed MW:	30kDa/45kDa

Synonyms:	BNIP3L, BNIP3a, NIX
Background:	This gene encodes a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The encoded protein binds to Bcl-2 and possesses the BH3 domain. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c.

UniProt Protein Function:	BNIP3L: Induces apoptosis. Interacts with viral and cellular anti-apoptosis proteins. Can overcome the suppressors BCL-2 and BCL-XL, although high levels of BCL-XL expression will inhibit apoptosis. Inhibits apoptosis induced by BNIP3. Involved in mitochondrial quality control via its interaction with SPATA18/MIEAP: in response to mitochondrial damage, participates to mitochondrial protein catabolic process (also named MALM) leading to the degradation of damaged proteins inside mitochondria. May function as a tumor suppressor. Self-associates. Interacts with BNIP3 and STEAP3. Interacts with SPATA18/MIEAP. Interacts with human adenovirus-2 E1B 19 kDa protein. Belongs to the NIP3 family.
UniProt Protein Details:	Protein type:Mitochondrial; Apoptosis; Membrane protein, integral; Nuclear envelope; Endoplasmic reticulum Chromosomal Location of Human Ortholog: 8p21 Cellular Component: endoplasmic reticulum; intrinsic to membrane; mitochondrial outer membrane; mitochondrion; nuclear envelope Molecular Function:identical protein binding; lamin binding; protein binding; protein heterodimerization activity; protein homodimerization activity Biological Process: defense response to virus; negative regulation of apoptosis; positive regulation of apoptosis; positive regulation of macroautophagy; regulation of apoptosis
NCBI Summary:	This gene encodes a protein that belongs to the pro-apoptotic subfamily within the Bcl-2 family of proteins. The encoded protein binds to Bcl-2 and possesses the BH3 domain. The protein directly targets mitochondria and causes apoptotic changes, including loss of membrane potential and the release of cytochrome c. [provided by RefSeq, Feb 2015]
UniProt Code:	O60238
NCBI GenInfo Identifier:	6093509
NCBI Gene ID:	665
NCBI Accession:	O60238.1
UniProt Secondary Accession:	O60238,Q5JW63, Q8NF87, B0AZS9,
UniProt Related Accession:	O60238
Molecular Weight:	19,559 Da
NCBI Full Name:	BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
NCBI Synonym Full Names:	BCL2 interacting protein 3 like
NCBI Official Symbol:	BNIP3L
NCBI Official Synonym Symbols:	NIX; BNIP3a
NCBI Protein Information:	BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
UniProt Protein Name:	BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like
UniProt Synonym Protein Names:	Adenovirus E1B19K-binding protein B5; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3A; NIP3-like protein X; NIP3L
Protein Family:	BCL2/adenovirus E1B 19 kDa protein-interacting protein
UniProt Gene Name:	BNIP3L
UniProt Entry Name:	BNI3L_HUMAN

	Western blot analysis of extracts of various cell lines, using BNIP3L antibody (CAB6283) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded human stomach using BNIP3L antibody (CAB6283) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat brain using BNIP3L antibody (CAB6283) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human lung cancer using BNIP3L antibody (CAB6283) at dilution of 1:100 (40x lens).

ELISA Kits

Human BNIP3L (BCL2/adenovirus E1B 19 kDa protein-interacting protein 3-like) ELISA Kit (HUFI07988)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)