Anti-BNIP3 Antibody (CAB5683)
- SKU:
- CAB5683
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Frequently bought together:
Description
| Antibody Name: | Anti-BNIP3 Antibody |
| Antibody SKU: | CAB5683 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human BNIP3 (NP_004043.3). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, NCI-H460, Jurkat, SH-SY5Y, Mouse kidney, Mouse heart, Mouse liver, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-164 of human BNIP3 (NP_004043.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGDA AADP PGPA LPCE FLRP GCGA PLSP GAQL GRGA PTSA FPPP AAEA HPAA RRGL RSPQ LPSG AMSQ NGAP GMQE ESLQ GSWV ELHF SNNG NGGS VPAS VSIY NGDM EKIL LDAQ HESG RSSS KSSH CDSP PRSQ TPQD TNRA SETD THSI GEKN SSQS EEDD |
| Gene ID: | 664 |
| Uniprot: | Q12983 |
| Cellular Location: | Mitochondrion, Mitochondrion outer membrane, Single-pass membrane protein |
| Calculated MW: | 27kDa |
| Observed MW: | 22-70kDa |
| Synonyms: | BNIP3, NIP3 |
| Background: | This gene is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The encoded protein interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation. |
| UniProt Protein Function: | BNIP3: Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2. Homodimer. Binds to BCL2. Interacts with BNIP3L and ACAA2. Also can interact with adenovirus E1B 19 kDa protein or Epstein-Barr virus BHRF1. Belongs to the NIP3 family. |
| UniProt Protein Details: | Protein type:Mitochondrial; Membrane protein, integral Chromosomal Location of Human Ortholog: 10q26.3 Cellular Component: nucleoplasm; mitochondrial outer membrane; mitochondrion; endoplasmic reticulum; dendrite; cytoplasm; mitochondrial membrane; nuclear envelope; nucleus; integral to mitochondrial outer membrane Molecular Function:identical protein binding; GTPase binding; protein binding; protein homodimerization activity; protein heterodimerization activity Biological Process: cell death; positive regulation of protein complex disassembly; induction of apoptosis by granzyme; viral reproduction; positive regulation of apoptosis; apoptosis; neuron apoptosis; positive regulation of programmed cell death; response to hyperoxia; brown fat cell differentiation; autophagic cell death; response to hypoxia; regulation of mitochondrial membrane permeability; mitochondrial fragmentation during apoptosis; negative regulation of membrane potential; defense response to virus; positive regulation of autophagy; negative regulation of apoptosis |
| NCBI Summary: | This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. This protein contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein encoded by this gene is known to induce apoptosis, even in the presence of BCL2. [provided by RefSeq, Mar 2011] |
| UniProt Code: | Q12983 |
| NCBI GenInfo Identifier: | 20532402 |
| NCBI Gene ID: | 664 |
| NCBI Accession: | Q12983.2 |
| UniProt Secondary Accession: | Q12983,O14620, Q96GP0, |
| UniProt Related Accession: | Q12983 |
| Molecular Weight: | 21,541 Da |
| NCBI Full Name: | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 |
| NCBI Synonym Full Names: | BCL2/adenovirus E1B 19kDa interacting protein 3 |
| NCBI Official Symbol: | BNIP3 |
| NCBI Official Synonym Symbols: | NIP3 |
| NCBI Protein Information: | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 |
| UniProt Protein Name: | BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 |
| Protein Family: | BNIP3 |
| UniProt Gene Name: | BNIP3 |
| UniProt Entry Name: | BNIP3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BNIP3 antibody (CAB5683) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Immunohistochemistry of paraffin-embedded mouse kidney using BNIP3 antibody (CAB5683) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat heart using BNIP3 antibody (CAB5683) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver damage using BNIP3 antibody (CAB5683) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using BNIP3 antibody (CAB5683) at dilution of 1:100. Blue: DAPI for nuclear staining. |
Related Products
Anti-BNIP3 Antibody (CAB15032)
