Anti-BNIP1 항체 (CAB7263)

Antibody Name:	Anti-BNIP1 Antibody
Antibody SKU:	CAB7263
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human BNIP1 (NP_001196.2).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse testis, Mouse liver, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human BNIP1 (NP_001196.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MAAP QDVH VRIC NQEI VKFD LEVK ALIQ DIRD CSGP LSAL TELN TKVK EKFQ QLRH RIQD LEQL AKEQ DKES EKQL LLQE VENH KKQM LSNQ ASWR KANL TCKI AIDN LEKA ELLQ GGDL LRQR KTTK ESLA QTSS TITE SLMG ISRM MAQQ VQQS EEAM QSLV TSSR TILD ANEE FKSM SGTI QLGR KLIT KYNR RELT
Gene ID:	662
Uniprot:	Q12981
Cellular Location:	Endoplasmic reticulum membrane, Mitochondrion, Single-pass type IV membrane protein
Calculated MW:	22kDa/26kDa/27kDa/31kDa
Observed MW:	26kDa

Synonyms:

BNIP1, NIP1, SEC20, TRG-8

Background:

This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. In addition, this protein is involved in vesicle transport into the endoplasmic reticulum. Alternative splicing of this gene results in four protein products with identical N- and C-termini.

UniProt Protein Function:	BNIP1: SNARE that may be involved in targeting and fusion of Golgi-derived retrograde transport vesicles with the ER. Required for maintenance of ER network. Implicated in the suppression of cell death. May be involved in mitochondrial autophagy. Belongs to the SEC20 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, integral; Apoptosis Chromosomal Location of Human Ortholog: 5q33-q34 Cellular Component: SNARE complex; mitochondrion; intracellular membrane-bound organelle; endoplasmic reticulum; cytoplasm; integral to endoplasmic reticulum membrane; nuclear envelope Molecular Function:SNAP receptor activity; protein binding Biological Process: endoplasmic reticulum organization and biogenesis; endoplasmic reticulum membrane fusion; vesicle-mediated transport; apoptosis; autophagy; negative regulation of apoptosis
NCBI Summary:	This gene is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein, which protects cells from virally-induced cell death. The encoded protein also interacts with E1B 19 kDa-like sequences of BCL2, another apoptotic protector. In addition, this protein is involved in vesicle transport into the endoplasmic reticulum. Alternative splicing of this gene results in four protein products with identical N- and C-termini. [provided by RefSeq, Mar 2011]
UniProt Code:	Q12981
NCBI GenInfo Identifier:	93141310
NCBI Gene ID:	662
NCBI Accession:	Q12981.3
UniProt Secondary Accession:	Q12981,O75622, O75623, O75624, Q6K044, Q96FG4, D3DQM3 D3DQM4, D3DQM5, D3DQM6,
UniProt Related Accession:	Q12981
Molecular Weight:	228
NCBI Full Name:	Vesicle transport protein SEC20
NCBI Synonym Full Names:	BCL2/adenovirus E1B 19kDa interacting protein 1
NCBI Official Symbol:	BNIP1
NCBI Official Synonym Symbols:	NIP1; SEC20; TRG-8
NCBI Protein Information:	vesicle transport protein SEC20; transformation-related gene 8 protein; BCL2/adenovirus E1B 19 kDa protein-interacting protein 1
UniProt Protein Name:	Vesicle transport protein SEC20
UniProt Synonym Protein Names:	BCL2/adenovirus E1B 19 kDa protein-interacting protein 1; Transformation-related gene 8 protein; TRG-8
Protein Family:	Vesicle transport protein
UniProt Gene Name:	BNIP1
UniProt Entry Name:	SEC20_HUMAN

	Western blot analysis of extracts of various cell lines, using BNIP1 Antibody (CAB7263) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using BNIP1 Antibody (CAB7263) at dilution of 1:100 (40x lens).

Antibodies

BNIP1 Antibody (PACO08032)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)