Anti-BLMH Antibody (CAB6535)
- SKU:
- CAB6535
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-BLMH Antibody |
| Antibody SKU: | CAB6535 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BLMH (NP_000377.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:20 - 1:50 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BLMH (NP_000377.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MSSS GLNS EKVA ALIQ KLNS DPQF VLAQ NVGT THDL LDIC LKRA TVQR AQHV FQHA VPQE GKPI TNQK SSGR CWIF SCLN VMRL PFMK KLNI EEFE FSQS YLFF WDKV ERCY FFLS AFVD TAQR KEPE DGRL VQFL LMNP ANDG GQWD MLVN IVEK YGVI PKKC FPES YTTE ATRR MNDI LNHK MREF CIRL RNLV HSGA TKGE ISAT QDVM MEEI FRVV CICL GNPP ETFT WEYR DKDK NYQK IGPI TPLE FYRE HVKP LFNM EDKI CLVN DPRP QHKY NKLY TVEY LSNM VGGR KTLY |
| Gene ID: | 642 |
| Uniprot: | Q13867 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 52kDa |
| Observed MW: | 50kDa |
| Synonyms: | BLMH, BH, BMH |
| Background: | Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. |
| UniProt Protein Function: | BLMH: The normal physiological role of BLM hydrolase is unknown, but it catalyzes the inactivation of the antitumor drug BLM (a glycopeptide) by hydrolyzing the carboxamide bond of its B- aminoalaninamide moiety thus protecting normal and malignant cells from BLM toxicity. Belongs to the peptidase C1 family. |
| UniProt Protein Details: | Protein type:EC 3.4.22.40; Protease Chromosomal Location of Human Ortholog: 17q11.2 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:aminopeptidase activity; carboxypeptidase activity; cysteine-type peptidase activity; protein binding Biological Process: protein polyubiquitination Disease: Alzheimer Disease |
| NCBI Summary: | Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer. The protein contains the signature active site residues of the cysteine protease papain superfamily. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q13867 |
| NCBI GenInfo Identifier: | 3023394 |
| NCBI Gene ID: | 642 |
| NCBI Accession: | Q13867.1 |
| UniProt Secondary Accession: | Q13867,Q53F86, Q9UER9, B2R796, |
| UniProt Related Accession: | Q13867 |
| Molecular Weight: | 52,562 Da |
| NCBI Full Name: | Bleomycin hydrolase |
| NCBI Synonym Full Names: | bleomycin hydrolase |
| NCBI Official Symbol: | BLMH |
| NCBI Official Synonym Symbols: | BH; BMH |
| NCBI Protein Information: | bleomycin hydrolase |
| UniProt Protein Name: | Bleomycin hydrolase |
| Protein Family: | Bleomycin hydrolase |
| UniProt Gene Name: | BLMH |
| UniProt Entry Name: | BLMH_HUMAN |
 | Western blot analysis of extracts of various cell lines, using BLMH Antibody (CAB6535) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of HeLa cells using BLMH antibody (CAB6535). Blue: DAPI for nuclear staining. |
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