Anti-beta 2 Microglobulin 항체 (CAB12404)

Antibody Name:	Anti-beta 2 Microglobulin Antibody [KO Validated]
Antibody SKU:	CAB12404
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IP
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin (NP_004039.1).

Application:	WB IHC IP
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IP 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, COS7, HeLa, K-562, Mouse liver, Mouse spleen

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 21-119 of human beta 2 Microglobulin (NP_004039.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IQRT PKIQ VYSR HPAE NGKS NFLN CYVS GFHP SDIE VDLL KNGE RIEK VEHS DLSF SKDW SFYL LYYT EFTP TEKD EYAC RVNH VTLS QPKI VKWD RDM
Gene ID:	567
Uniprot:	P61769
Cellular Location:	Secreted
Calculated MW:	13kDa
Observed MW:	12kDa

Synonyms:

B2M, IMD43

Background:

This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

UniProt Protein Function:	B2M: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Heterodimer of an alpha chain and a beta chain. Beta-2- microglobulin is the beta-chain of major histocompatibility complex class I molecules. Polymers of beta 2-microglobulin can be found in tissues from patients on long-term hemodialysis. Belongs to the beta-2-microglobulin family.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 15q21.1 Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; phagocytic vesicle membrane; focal adhesion; membrane; early endosome membrane; endoplasmic reticulum lumen; cytoplasm; plasma membrane; extracellular region; MHC class I protein complex; external side of plasma membrane Molecular Function:identical protein binding; protein binding Biological Process: response to drug; regulation of immune response; viral reproduction; positive regulation of T cell mediated cytotoxicity; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; cytokine and chemokine mediated signaling pathway; T cell differentiation in the thymus; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; regulation of defense response to virus by virus; retinal homeostasis; iron ion homeostasis; antigen processing and presentation of peptide antigen via MHC class I; response to cadmium ion; response to molecule of bacterial origin; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; protein refolding; antigen processing and presentation of exogenous protein antigen via MHC class Ib, TAP-dependent Disease: Hypoproteinemia, Hypercatabolic
NCBI Summary:	This gene encodes a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.[provided by RefSeq, Aug 2014]
UniProt Code:	P61769
NCBI GenInfo Identifier:	48428791
NCBI Gene ID:	567
NCBI Accession:	P61769.1
UniProt Related Accession:	P61769
Molecular Weight:
NCBI Full Name:	Beta-2-microglobulin
NCBI Synonym Full Names:	beta-2-microglobulin
NCBI Official Symbol:	B2M
NCBI Official Synonym Symbols:	IMD43
NCBI Protein Information:	beta-2-microglobulin
UniProt Protein Name:	Beta-2-microglobulin
Protein Family:	Beta-2-microglobulin
UniProt Gene Name:	B2M
UniProt Entry Name:	B2MG_HUMAN

	Western blot analysis of extracts of various cell lines, using beta 2 Microglobulin antibody (CAB12404) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
	Western blot analysis of extracts from normal (control) and beta 2 Microglobulin knockout (KO) 293T cells, using beta 2 Microglobulin antibody (CAB12404) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded rat brain using beta 2 Microglobulin antibody (CAB12404) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human prostate using beta 2 Microglobulin antibody (CAB12404) at dilution of 1:100 (40x lens).

Antibodies	ELISA Kits
Anti-beta 2 Microglobulin Antibody (CAB1562)	Human Beta 2-microglobulin ELISA Kit
Anti-beta 2 Microglobulin Antibody (CAB11642)	Human Beta-2-microglobulin (B2M) ELISA Kit
B2M Antibody (PACO07959)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)