Anti-BCAT1 Antibody (CAB16351)
- SKU:
- CAB16351
- Product type:
- Antibody
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-BCAT1 Antibody |
| Antibody SKU: | CAB16351 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide of human BCAT1 |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Rat |
| Positive Samples: | rat brain |
| Immunogen: | A synthetic peptide of human BCAT1 |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | Email for sequence |
| Gene ID: | 586 |
| Uniprot: | P54687 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 36kDa/38kDa/42kDa/44kDa |
| Observed MW: | Refer to figures |
| Synonyms: | BCAT1, BCATC, BCT1, ECA39, MECA39, PNAS121, PP18 |
| Background: | This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. |
| UniProt Protein Function: | BCAT1: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:EC 2.6.1.42; Amino Acid Metabolism - valine, leucine and isoleucine biosynthesis; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Transferase Chromosomal Location of Human Ortholog: 12p12.1 Cellular Component: mitochondrion; cytosol Molecular Function:identical protein binding; branched-chain-amino-acid transaminase activity Biological Process: cell proliferation; branched chain family amino acid catabolic process; branched chain family amino acid biosynthetic process; G1/S transition of mitotic cell cycle |
| NCBI Summary: | This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010] |
| UniProt Code: | P54687 |
| NCBI GenInfo Identifier: | 215274162 |
| NCBI Gene ID: | 586 |
| NCBI Accession: | P54687.3 |
| UniProt Secondary Accession: | P54687,Q68DQ7, Q96MY9, B3KY27, B7Z2M5, B7Z5L0, F5H5E4 |
| UniProt Related Accession: | P54687 |
| Molecular Weight: | 386 |
| NCBI Full Name: | Branched-chain-amino-acid aminotransferase, cytosolic |
| NCBI Synonym Full Names: | branched chain amino-acid transaminase 1, cytosolic |
| NCBI Official Symbol: | BCAT1 |
| NCBI Official Synonym Symbols: | BCT1; PP18; BCATC; ECA39; MECA39; PNAS121 |
| NCBI Protein Information: | branched-chain-amino-acid aminotransferase, cytosolic; placental protein 18; branched chain aminotransferase 1, cytosolic |
| UniProt Protein Name: | Branched-chain-amino-acid aminotransferase, cytosolic |
| UniProt Synonym Protein Names: | Protein ECA39 |
| Protein Family: | Branched-chain-amino-acid aminotransferase |
| UniProt Gene Name: | BCAT1 |
| UniProt Entry Name: | BCAT1_HUMAN |
 | Western blot analysis of extracts of rat brain, using BCAT1 antibody (CAB16351) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
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