Anti-B4GALT4 Antibody (CAB14693)
- SKU:
- CAB14693
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-B4GALT4 Antibody |
| Antibody SKU: | CAB14693 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 35-344 of human B4GALT4 (NP_003769.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, HT-29, Mouse kidney, Mouse thymus, Mouse lung, Rat kidney, Rat thymus, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 35-344 of human B4GALT4 (NP_003769.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VGAI QEIP KAKE FMAN FHKT LILG KGKT LTNE ASTK KVEL DNCP SVSP YLRG QSKL IFKP DLTL EEVQ AENP KVSR GRYR PQEC KALQ RVAI LVPH RNRE KHLM YLLE HLHP FLQR QQLD YGIY VIHQ AEGK KFNR AKLL NVGY LEAL KEEN WDCF IFHD VDLV PEND FNLY KCEE HPKH LVVG RNST GYRL RYSG YFGG VTAL SREQ FFKV NGFS NNYW GWGG EDDD LRLR VELQ RMKI SRPL PEVG KYTM VFHT RDKG NEVN AERM KLLH QVSR VWRT DGLS SCSY KLVS VEHN PLYI NITV DFWF GA |
| Gene ID: | 8702 |
| Uniprot: | O60513 |
| Cellular Location: | Golgi apparatus, Golgi stack membrane, Single-pass type II membrane protein |
| Calculated MW: | 40kDa |
| Observed MW: | 40kDa |
| Synonyms: | B4GALT4, B4Gal-T4, beta4Gal-T4, beta-1 |
| Background: | This gene is one of seven beta-1, 4-galactosyltransferase (beta4GalT) genes. They encode type II membrane-bound glycoproteins that appear to have exclusive specificity for the donor substrate UDP-galactose; all transfer galactose in a beta1, 4 linkage to similar acceptor sugars: GlcNAc, Glc, and Xyl. Each beta4GalT has a distinct function in the biosynthesis of different glycoconjugates and saccharide structures. As type II membrane proteins, they have an N-terminal hydrophobic signal sequence that directs the protein to the Golgi apparatus and which then remains uncleaved to function as a transmembrane anchor. By sequence similarity, the beta4GalTs form four groups: beta4GalT1 and beta4GalT2, beta4GalT3 and beta4GalT4, beta4GalT5 and beta4GalT6, and beta4GalT7. The enzyme encoded by this gene appears to mainly play a role in glycolipid biosynthesis. Two alternatively spliced transcript variants have been found for this gene. |
| UniProt Code: | O60513 |
| NCBI GenInfo Identifier: | 13123971 |
| NCBI Gene ID: | 8702 |
| NCBI Accession: | O60513.1 |
| UniProt Secondary Accession: | O60513,Q68D68, Q9BSW3, Q9C078, |
| UniProt Related Accession: | O60513 |
| Molecular Weight: | 344 |
| NCBI Full Name: | Beta-1,4-galactosyltransferase 4 |
| NCBI Synonym Full Names: | UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 4 |
| NCBI Official Symbol: | B4GALT4 |
| NCBI Official Synonym Symbols: | B4Gal-T4; beta4Gal-T4 |
| NCBI Protein Information: | beta-1,4-galactosyltransferase 4; beta-1,4-GalTase 4; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4; UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase 4; beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase 4; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4 |
| UniProt Protein Name: | Beta-1,4-galactosyltransferase 4 |
| UniProt Synonym Protein Names: | UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 4; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 4Including the following 2 domains:N-acetyllactosamine synthase (EC:2.4.1.90)Alternative name(s):Nal synthase |
| Protein Family: | Beta-1,4-galactosyltransferase |
| UniProt Gene Name: | B4GALT4 |
| UniProt Entry Name: | B4GT4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using B4GALT4 antibody (CAB14693) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunofluorescence analysis of HeLa cells using B4GALT4 antibody (CAB14693) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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