Anti-B3GNT4 Antibody (CAB15896)
- SKU:
- CAB15896
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-B3GNT4 Antibody |
| Antibody SKU: | CAB15896 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 165-305 of human B3GNT4 (NP_110392.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Rat brain, Rat intestine |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 165-305 of human B3GNT4 (NP_110392.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | APPA QLLA YESR EFDD ILQW DFTE DFFN LTLK ELHL QRWV VAAC PQAH FMLK GDDD VFVH VPNV LEFL DGWD PAQD LLVG DVIR QALP NRNT KVKY FIPP SMYR ATHY PPYA GGGG YVMS RATV RRLQ AIME DAEL FPID D |
| Gene ID: | 79369 |
| Uniprot: | Q9C0J1 |
| Cellular Location: | Golgi apparatus membrane, Single-pass type II membrane protein |
| Calculated MW: | 39kDa/42kDa |
| Observed MW: | 42kDa |
| Synonyms: | B3GNT4, B3GN-T4 |
| Background: | This gene encodes a member of the beta-1, 3-N-acetylglucosaminyltransferase protein family. The encoded enzyme is involved in the biosynthesis of poly-N-acetyllactosamine chains and prefers lacto-N-neotetraose as a substrate. It is a type II transmembrane protein. |
| UniProt Protein Function: | B3GNT4: Has a beta-1,3-N-acetylglucosaminyltransferase activity for type 2 oligosaccharides. Belongs to the glycosyltransferase 31 family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; EC 2.4.1.-; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Transferase Chromosomal Location of Human Ortholog: 12q24 Cellular Component: Golgi membrane Molecular Function:N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity; UDP-galactose:beta-N-acetylglucosamine beta-1,3-galactosyltransferase activity Biological Process: keratan sulfate biosynthetic process; O-glycan processing; poly-N-acetyllactosamine biosynthetic process |
| NCBI Summary: | This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase protein family. The encoded enzyme is involved in the biosynthesis of poly-N-acetyllactosamine chains and prefers lacto-N-neotetraose as a substrate. It is a type II transmembrane protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9C0J1 |
| NCBI GenInfo Identifier: | 74752494 |
| NCBI Gene ID: | 79369 |
| NCBI Accession: | Q9C0J1.1 |
| UniProt Secondary Accession: | Q9C0J1,Q8N5W4, Q8N934, Q8ND21, Q8WWR5, Q8WY02, Q96QH5 |
| UniProt Related Accession: | Q9C0J1 |
| Molecular Weight: | 39,848 Da |
| NCBI Full Name: | N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 4 |
| NCBI Synonym Full Names: | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4 |
| NCBI Official Symbol: | B3GNT4 |
| NCBI Official Synonym Symbols: | B3GN-T4; beta3Gn-T4 |
| NCBI Protein Information: | N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 4 |
| UniProt Protein Name: | N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase 4 |
| UniProt Synonym Protein Names: | UDP-GlcNAc:betaGal beta-1,3-N-acetylglucosaminyltransferase 4; BGnT-4; Beta-1,3-Gn-T4; Beta-1,3-N-acetylglucosaminyltransferase 4; Beta3Gn-T4 |
| Protein Family: | N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase |
| UniProt Gene Name: | B3GNT4 |
| UniProt Entry Name: | B3GN4_HUMAN |
 | Western blot analysis of extracts of various cell lines, using B3GNT4 antibody (CAB15896) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of C6 cells using B3GNT4 antibody (CAB15896) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of HeLa cells using B3GNT4 antibody (CAB15896) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using B3GNT4 antibody (CAB15896) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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