Anti-AURKA Antibody (CAB15728)
- SKU:
- CAB15728
- Product type:
- Antibody
- Reactivity:
- Human
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-AURKA Antibody |
| Antibody SKU: | CAB15728 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human AURKA (NP_940837.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human |
| Positive Samples: | HeLa, MCF7, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-130 of human AURKA (NP_940837.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDRS KENC ISGP VKAT APVG GPKR VLVT QQFP CQNP LPVN SGQA QRVL CPSN SSQR IPLQ AQKL VSSH KPVQ NQKQ KQLQ ATSV PHPV SRPL NNTQ KSKQ PLPS APEN NPEE ELAS KQKN EESK KRQW AL |
| Gene ID: | 6790 |
| Uniprot: | O14965 |
| Cellular Location: | Cytoplasm, centriole, centrosome, cilium basal body, cytoskeleton, microtubule organizing center, spindle pole |
| Calculated MW: | 45kDa |
| Observed MW: | 45kDa |
| Synonyms: | AURKA, AIK, ARK1, AURA, BTAK, PPP1R47, STK15, STK6, STK7 |
| Background: | The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. |
| UniProt Protein Function: | AurA: a member of the AUR family of kinases. A cell cycle-regulated serine/threonine protein kinase that is overexpressed in many tumor cell lines. Regulated by phosphorylation-dependent proteasomal degradation. Localized to mitotic centrosomes and spindle microtubules and is required for centrosome maturation. Loss of Aurora A leads to defective mitotic spindles and gross errors in chromosome segregation. Required for centrosome duplication and chromosome segregation. Overexpression in culture drives transformation and aneuploidy, and negatively regulates p53. Amplified or overexpressed in many tumors or cell lines. Found as a skin tumor susceptibility gene in mouse, and a human SNP in a degradation domain is weakly cancer-associated and undergoes allele-specific amplification. Inhibitors: VX-680, ZM447439, Hesperadin, SNS-595. |
| UniProt Protein Details: | Protein type:Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Oncoprotein; Kinase, protein; Other group; AUR family Chromosomal Location of Human Ortholog: 20q13 Cellular Component: microtubule cytoskeleton; germinal vesicle; axon hillock; centrosome; pronucleus; spindle microtubule; perinuclear region of cytoplasm; spindle; midbody; spindle midzone; spindle pole centrosome; cytosol; nucleus; condensed nuclear chromosome, pericentric region Molecular Function:protein serine/threonine kinase activity; protein binding; ubiquitin protein ligase binding; protein serine/threonine/tyrosine kinase activity; histone serine kinase activity; protein kinase binding; ATP binding; protein kinase activity Biological Process: spindle stabilization; mitosis; regulation of centrosome cycle; positive regulation of mitosis; protein amino acid autophosphorylation; regulation of protein stability; regulation of cytokinesis; protein amino acid phosphorylation; mitotic centrosome separation; mitotic spindle organization and biogenesis; centrosome localization; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; cell division; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; mitotic cell cycle; G2/M transition of mitotic cell cycle; negative regulation of protein binding; spindle assembly involved in female meiosis I; negative regulation of apoptosis; anterior/posterior axis specification Disease: Colorectal Cancer |
| NCBI Summary: | The protein encoded by this gene is a cell cycle-regulated kinase that appears to be involved in microtubule formation and/or stabilization at the spindle pole during chromosome segregation. The encoded protein is found at the centrosome in interphase cells and at the spindle poles in mitosis. This gene may play a role in tumor development and progression. A processed pseudogene of this gene has been found on chromosome 1, and an unprocessed pseudogene has been found on chromosome 10. Multiple transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | O14965 |
| NCBI GenInfo Identifier: | 27923855 |
| NCBI Gene ID: | 6790 |
| NCBI Accession: | O14965.2 |
| UniProt Secondary Accession: | O14965,O60445, O75873, Q9BQD6, Q9UPG5, E1P5F9, |
| UniProt Related Accession: | O14965 |
| Molecular Weight: | 403 |
| NCBI Full Name: | Aurora kinase A |
| NCBI Synonym Full Names: | aurora kinase A |
| NCBI Official Symbol: | AURKA |
| NCBI Official Synonym Symbols: | AIK; ARK1; AURA; BTAK; STK6; STK7; STK15; AURORA2; PPP1R47 |
| NCBI Protein Information: | aurora kinase A; ARK-1; hARK1; aurora 2; Aurora-A kinase; IPL1-related kinase; aurora-related kinase 1; aurora/IPL1-like kinase; serine/threonine kinase 6; aurora/IPL1-related kinase 1; breast tumor-amplified kinase; breast-tumor-amplified kinase; serine/threonine-protein kinase 6; serine/threonine protein kinase 15; serine/threonine-protein kinase 15; serine/threonine-protein kinase aurora-A; protein phosphatase 1, regulatory subunit 47 |
| UniProt Protein Name: | Aurora kinase A |
| UniProt Synonym Protein Names: | Aurora 2; Aurora/IPL1-related kinase 1; ARK-1; Aurora-related kinase 1; hARK1; Breast tumor-amplified kinase; Serine/threonine-protein kinase 15; Serine/threonine-protein kinase 6; Serine/threonine-protein kinase aurora-A |
| Protein Family: | Aurora kinase |
| UniProt Gene Name: | AURKA |
| UniProt Entry Name: | AURKA_HUMAN |
 | Western blot analysis of extracts of various cell lines, using AURKA antibody (CAB15728) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of U2OS cells using AURKA antibody (CAB15728) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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