Anti-ATPIF1 Antibody (CAB5099)
- SKU:
- CAB5099
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ATPIF1 Antibody |
| Antibody SKU: | CAB5099 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-106 of human ATPIF1 (NP_057395.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse |
| Positive Samples: | MCF-7, 22Rv1, HeLa, H460, Mouse brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 26-106 of human ATPIF1 (NP_057395.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | GSDQ SENV DRGA GSIR EAGG AFGK REQA EEER YFRA QSRE QLAA LKKH HEEE IVHH KKEI ERLQ KEIE RHKQ KIKM LKHD D |
| Gene ID: | 93974 |
| Uniprot: | Q9UII2 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 6kDa/7kDa/12kDa |
| Observed MW: | 12kDa |
| Synonyms: | ATPIF1, ATPI, ATPIP, IP |
| Background: | This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. |
| UniProt Protein Function: | ATPIF1: Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Homodimer at a pH value below 6.5, inactive homotetramer above. Belongs to the ATPase inhibitor family. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Mitochondrial; Inhibitor Chromosomal Location of Human Ortholog: 1p35.3 Cellular Component: cell surface; mitochondrial proton-transporting ATP synthase complex; mitochondrion Molecular Function:5-formyltetrahydrofolate cyclo-ligase activity; angiostatin binding; ATPase binding; ATPase inhibitor activity; calmodulin binding; enzyme inhibitor activity; protein homodimerization activity Biological Process: angiogenesis; erythrocyte differentiation; folic acid and derivative biosynthetic process; generation of precursor metabolites and energy; heme biosynthetic process; mitochondrial depolarization; negative regulation of ATPase activity; negative regulation of endothelial cell proliferation; negative regulation of hydrolase activity; protein homooligomerization; protein homotetramerization |
| NCBI Summary: | This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9UII2 |
| NCBI GenInfo Identifier: | 12585262 |
| NCBI Gene ID: | 93974 |
| NCBI Accession: | Q9UII2.1 |
| UniProt Secondary Accession: | Q9UII2,Q5JXL8, Q6IAQ7, Q9BSL9, |
| UniProt Related Accession: | Q9UII2 |
| Molecular Weight: | |
| NCBI Full Name: | ATPase inhibitor, mitochondrial |
| NCBI Synonym Full Names: | ATPase inhibitory factor 1 |
| NCBI Official Symbol: | ATPIF1 |
| NCBI Official Synonym Symbols: | IP; ATPI; ATPIP |
| NCBI Protein Information: | ATPase inhibitor, mitochondrial |
| UniProt Protein Name: | ATPase inhibitor, mitochondrial |
| UniProt Synonym Protein Names: | Inhibitor of F(1)F(o)-ATPase; IF(1); IF1 |
| Protein Family: | ATPase inhibitor |
| UniProt Gene Name: | ATPIF1 |
| UniProt Entry Name: | ATIF1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATPIF1 antibody (CAB5099) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded human stomach using ATPIF1 antibody (CAB5099) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human liver cancer using ATPIF1 antibody (CAB5099) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human trachea using ATPIF1 antibody (CAB5099) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using ATPIF1 antibody (CAB5099) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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