Anti-ATPIF1 Antibody (CAB19946)[KO Validated]

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SKU:
CAB19946
Product type:
Antibody
Reactivity:
Human
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Biology
Frequently bought together:

Description

system_update_alt데이터시트system_update_alt안전 데이터시트
Antibody Name:Anti-ATPIF1 Antibody [KO Validated]
Antibody SKU:CAB19946
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human ATPIF1.
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:Human, Mouse
Positive Samples:293T
Immunogen:Recombinant protein of human ATPIF1.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:93974
Uniprot:Q9UII2
Cellular Location:Mitochondrion
Calculated MW:6kDa/7kDa/12kDa
Observed MW:12KDa
Anti-ATPIF1 Antibody [KO Validated] (CAB19946)Western blot analysis of extracts from normal (control) and ATPIF1 knockout (KO) 293T cells, using ATPIF1 antibody (CAB19946) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:ATPIF1, ATPI, ATPIP, IP
Background:This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified.
UniProt Protein Function:ATPIF1: Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix. Homodimer at a pH value below 6.5, inactive homotetramer above. Belongs to the ATPase inhibitor family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Mitochondrial; Inhibitor

Chromosomal Location of Human Ortholog: 1p35.3

Cellular Component: cell surface; mitochondrial proton-transporting ATP synthase complex; mitochondrion

Molecular Function:5-formyltetrahydrofolate cyclo-ligase activity; angiostatin binding; ATPase binding; ATPase inhibitor activity; calmodulin binding; enzyme inhibitor activity; protein homodimerization activity

Biological Process: angiogenesis; erythrocyte differentiation; folic acid and derivative biosynthetic process; generation of precursor metabolites and energy; heme biosynthetic process; mitochondrial depolarization; negative regulation of ATPase activity; negative regulation of endothelial cell proliferation; negative regulation of hydrolase activity; protein homooligomerization; protein homotetramerization

NCBI Summary:This gene encodes a mitochondrial ATPase inhibitor. Alternative splicing occurs at this locus and three transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UII2
NCBI GenInfo Identifier:12585262
NCBI Gene ID:93974
NCBI Accession:Q9UII2.1
UniProt Secondary Accession:Q9UII2,Q5JXL8, Q6IAQ7, Q9BSL9,
UniProt Related Accession:Q9UII2
Molecular Weight:
NCBI Full Name:ATPase inhibitor, mitochondrial
NCBI Synonym Full Names:ATPase inhibitory factor 1
NCBI Official Symbol:ATPIF1  
NCBI Official Synonym Symbols:IP; ATPI; ATPIP  
NCBI Protein Information:ATPase inhibitor, mitochondrial
UniProt Protein Name:ATPase inhibitor, mitochondrial
UniProt Synonym Protein Names:Inhibitor of F(1)F(o)-ATPase; IF(1); IF1
Protein Family:ATPase inhibitor
UniProt Gene Name:ATPIF1  
UniProt Entry Name:ATIF1_HUMAN

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