Anti-ATPAF2 Antibody (CAB15928)
- SKU:
- CAB15928
- Product type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-ATPAF2 Antibody |
| Antibody SKU: | CAB15928 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 41-200 of human ATPAF2 (NP_663729.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse heart, Mouse kidney, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 41-200 of human ATPAF2 (NP_663729.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | APPT ERKR FYQN VSIT QGEG GFEI NLDH RKLK TPQA KLFT VPSE ALAI AVAT EWDS QQDT IKYY TMHL TTLC NTSL DNPT QRNK DQLI RAAV KFLD TDTI CYRV EEPE TLVE LQRN EWDP IIEW AEKR YGVE ISSS TSIM GPSI PAKT REVL VSHL ASYN |
| Gene ID: | 91647 |
| Uniprot: | Q8N5M1 |
| Cellular Location: | Mitochondrion |
| Calculated MW: | 32kDa |
| Observed MW: | 38kDa |
| Synonyms: | ATPAF2, ATP12, ATP12p, LP3663, MC5DN1 |
| Background: | This gene encodes an assembly factor for the F(1) component of the mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. This gene is located within the Smith-Magenis syndrome region on chromosome 17. An alternatively spliced transcript variant has been described, but its biological validity has not been determined. |
| UniProt Protein Function: | ATPAF2: May play a role in the assembly of the F1 component of the mitochondrial ATP synthase (ATPase). Defects in ATPAF2 are a cause of mitochondrial complex V deficiency nuclear type 1 (MC5DN1). A mitochondrial disorder with heterogeneous clinical manifestations including dysmorphic features, psychomotor retardation, hypotonia, growth retardation, cardiomyopathy, enlarged liver, hypoplastic kidneys and elevated lactate levels in urine, plasma and cerebrospinal fluid. Belongs to the ATP12 family. |
| UniProt Protein Details: | Protein type:Mitochondrial Chromosomal Location of Human Ortholog: 17p11.2 Cellular Component: cytosol; nuclear speck Molecular Function:protein binding Disease: Mitochondrial Complex V (atp Synthase) Deficiency, Nuclear Type 1 |
| NCBI Summary: | This gene encodes an assembly factor for the F(1) component of the mitochondrial ATP synthase. This protein binds specifically to the F1 alpha subunit and is thought to prevent this subunit from forming nonproductive homooligomers during enzyme assembly. This gene is located within the Smith-Magenis syndrome region on chromosome 17. An alternatively spliced transcript variant has been described, but its biological validity has not been determined. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q8N5M1 |
| NCBI GenInfo Identifier: | 21735485 |
| NCBI Gene ID: | 91647 |
| NCBI Accession: | NP_663729.1 |
| UniProt Secondary Accession: | Q8N5M1,Q6XYC7, A6NDE5, A8K2J2, |
| UniProt Related Accession: | Q8N5M1 |
| Molecular Weight: | 32.6kD |
| NCBI Full Name: | ATP synthase mitochondrial F1 complex assembly factor 2 |
| NCBI Synonym Full Names: | ATP synthase mitochondrial F1 complex assembly factor 2 |
| NCBI Official Symbol: | ATPAF2 |
| NCBI Official Synonym Symbols: | ATP12; ATP12p; LP3663; MC5DN1 |
| NCBI Protein Information: | ATP synthase mitochondrial F1 complex assembly factor 2 |
| UniProt Protein Name: | ATP synthase mitochondrial F1 complex assembly factor 2 |
| UniProt Synonym Protein Names: | ATP12 homolog |
| Protein Family: | ATP synthase |
| UniProt Gene Name: | ATPAF2 |
 | Western blot analysis of extracts of various cell lines, using ATPAF2 antibody (CAB15928) at 1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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