Anti-ATP7B Antibody (CAB5676)
- SKU:
- CAB5676
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ATP7B Antibody |
| Antibody SKU: | CAB5676 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1055-1354 of human ATP7B (NP_001230111.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | BT-474, HL-60, Mouse liver |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1055-1354 of human ATP7B (NP_001230111.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | SDAM TDHE MKGQ TAIL VAID GVLC GMIA IADA VKQE AALA VHTL QSMG VDVV LITG DNRK TARA IATQ VGIN KVFA EVLP SHKV AKVQ ELQN KGKK VAMV GDGV NDSP ALAQ ADMG VAIG TGTD VAIE AADV VLIR NDLL DVVA SIHL SKRT VRRI RINL VLAL IYNL VGIP IAAG VFMP IGIV LQPW MGSA AMAA SSVS VVLS SLQL KCYK KPDL ERYE AQAH GHMK PLTA SQVS VHIG MDDR WRDS PRAT PWDQ VSYV SQVS LSSL TSDK PSRH SAAA DDDG DKWS LLLN GRDE EQYI |
| Gene ID: | 540 |
| Uniprot: | P35670 |
| Cellular Location: | Cytoplasm, Golgi apparatus, Golgi apparatus membrane, Mitochondrion, Multi-pass membrane protein, Multi-pass membrane protein, trans-Golgi network membrane |
| Calculated MW: | 133kDa/145kDa/155kDa/157kDa |
| Observed MW: | 150kDa |
| Synonyms: | ATP7B, PWD, WC1, WD, WND |
| Background: | This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). |
| UniProt Protein Function: | ATP7B: Involved in the export of copper out of the cells, such as the efflux of hepatic copper into the bile. Monomer. Interacts with COMMD1/MURR1. Most abundant in liver and kidney and also found in brain. Isoform 2 is expressed in brain but not in liver. The cleaved form WND/140 kDa is found in liver cell lines and other tissues. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IB subfamily. 4 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, integral; Transporter, ion channel; Membrane protein, multi-pass; EC 3.6.3.54; Vesicle; Transporter; Hydrolase Chromosomal Location of Human Ortholog: 13q14.3 Cellular Component: basolateral plasma membrane; cytoplasmic membrane-bound vesicle; Golgi membrane; integral to plasma membrane; late endosome; membrane; mitochondrion; perinuclear region of cytoplasm; tight junction; trans-Golgi network Molecular Function:ATP binding; copper ion binding; copper-exporting ATPase activity; protein binding; zinc ion binding Biological Process: cellular copper ion homeostasis; cellular zinc ion homeostasis; circadian rhythm; copper ion export; copper ion import; copper ion transport; intracellular copper ion transport; lactation; metabolic process; response to cAMP; response to copper ion; response to zinc ion; sequestering of calcium ion; transmembrane transport Disease: Wilson Disease |
| NCBI Summary: | This gene is a member of the P-type cation transport ATPase family and encodes a protein with several membrane-spanning domains, an ATPase consensus sequence, a hinge domain, a phosphorylation site, and at least 2 putative copper-binding sites. This protein functions as a monomer, exporting copper out of the cells, such as the efflux of hepatic copper into the bile. Alternate transcriptional splice variants, encoding different isoforms with distinct cellular localizations, have been characterized. Mutations in this gene have been associated with Wilson disease (WD). [provided by RefSeq, Jul 2008] |
| UniProt Code: | P35670 |
| NCBI GenInfo Identifier: | 239938919 |
| NCBI Gene ID: | 540 |
| NCBI Accession: | P35670.4 |
| UniProt Secondary Accession: | P35670,Q16318, Q16319, Q4U3V3, Q59FJ9, Q5T7X7, |
| UniProt Related Accession: | P35670 |
| Molecular Weight: | 155,125 Da |
| NCBI Full Name: | Copper-transporting ATPase 2 |
| NCBI Synonym Full Names: | ATPase copper transporting beta |
| NCBI Official Symbol: | ATP7B |
| NCBI Official Synonym Symbols: | WD; PWD; WC1; WND |
| NCBI Protein Information: | copper-transporting ATPase 2 |
| UniProt Protein Name: | Copper-transporting ATPase 2 |
| UniProt Synonym Protein Names: | Copper pump 2; Wilson disease-associated protein |
| UniProt Gene Name: | ATP7B |
| UniProt Entry Name: | ATP7B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATP7B antibody (CAB5676) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of U2OS cells using ATP7B antibody (CAB5676). Blue: DAPI for nuclear staining. |
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