Anti-ATP6AP1 항체 (CAB1209)

Antibody Name:	Anti-ATP6AP1 Antibody
Antibody SKU:	CAB1209
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 90-390 of human ATP6AP1 (NP_001174.2).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse
Positive Samples:	THP-1, OVCAR3, PC-3, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 90-390 of human ATP6AP1 (NP_001174.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LLFL QDKL SIED FTAY GGVF GNKQ DSAF SNLE NALD LAPS SLVL PAVD WYAV STLT TYLQ EKLG ASPL HVDL ATLR ELKL NASL PALL LIRL PYTA SSGL MAPR EVLT GNDE VIGQ VLST LKSE DVPY TAAL TAVR PSRV ARDV AVVA GGLG RQLL QKQP VSPV IHPP VSYN DTAP RILF WAQN FSVA YKDQ WEDL TPLT FGVQ ELNL TGSF WNDS FARL SLTY ERLF GTTV TFKF ILAN RLYP VSAR HWFT MERL EVHS NGSV AYFN ASQV TGPS IYSF HCEY VSSL SKKG SLLV ARTQ P
Gene ID:	537
Uniprot:	Q15904
Cellular Location:	Single-pass membrane protein, Vacuole membrane
Calculated MW:	52kDa
Observed MW:	50kDa

Synonyms:

ATP6AP1, 16A, ATP6IP1, ATP6S1, Ac45, CF2, VATPS1, XAP-3, XAP3

Background:

This gene encodes a component of a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. This protein may also play a role in early development.

UniProt Protein Function:	ATP6AP1: Vacuolar ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells. Belongs to the vacuolar ATPase subunit S1 family.
UniProt Protein Details:	Protein type:Transporter; EC 3.6.3.14; Energy Metabolism - oxidative phosphorylation; Hydrolase; Membrane protein, integral Chromosomal Location of Human Ortholog: Xq28 Cellular Component: vacuolar membrane; integral to membrane; endosome membrane; proton-transporting two-sector ATPase complex Molecular Function:transporter activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; hydrogen ion transporting ATPase activity, rotational mechanism; Rab GTPase binding; ATP binding Biological Process: positive regulation of osteoblast differentiation; proton transport; cellular iron ion homeostasis; ATP hydrolysis coupled proton transport; pH reduction; insulin receptor signaling pathway; positive regulation of bone resorption; transferrin transport; transmembrane transport; establishment of organelle localization; positive regulation of exocytosis
NCBI Summary:	This gene encodes a component of a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. Vacuolar ATPase (V-ATPase) is comprised of a cytosolic V1 (site of the ATP catalytic site) and a transmembrane V0 domain. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, and receptor-mediated endocytosis. The encoded protein of this gene may assist in the V-ATPase-mediated acidification of neuroendocrine secretory granules. This protein may also play a role in early development. [provided by RefSeq, Aug 2013]
UniProt Code:	Q15904
NCBI GenInfo Identifier:	12230759
NCBI Gene ID:	537
NCBI Accession:	Q15904.2
UniProt Secondary Accession:	Q15904,Q8NBT4, Q9H0C7, A6ZKI4,
UniProt Related Accession:	Q15904
Molecular Weight:	470
NCBI Full Name:	V-type proton ATPase subunit S1
NCBI Synonym Full Names:	ATPase, H+ transporting, lysosomal accessory protein 1
NCBI Official Symbol:	ATP6AP1
NCBI Official Synonym Symbols:	16A; CF2; Ac45; XAP3; XAP-3; ATP6S1; VATPS1; ATP6IP1
NCBI Protein Information:	V-type proton ATPase subunit S1; protein XAP-3; H-ATPase subunit; V-ATPase subunit S1; V-ATPase Ac45 subunit; V-ATPase S1 accessory protein; vacuolar proton pump subunit S1; ATPase, H+ transporting, lysosomal interacting protein 1; ATPase, H+ transporting, lysosomal (vacuolar proton pump), subunit 1
UniProt Protein Name:	V-type proton ATPase subunit S1
UniProt Synonym Protein Names:	Protein XAP-3; V-ATPase Ac45 subunit; V-ATPase S1 accessory protein; Vacuolar proton pump subunit S1
Protein Family:	V-type proton ATPase
UniProt Gene Name:	ATP6AP1
UniProt Entry Name:	VAS1_HUMAN

Western blot analysis of extracts of various cell lines, using ATP6AP1 antibody (CAB1209) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Antibodies

ATP6AP1 Antibody (PACO07441)

ATP6AP1 Antibody (PACO07931)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)