Anti-ATP5L Antibody (CAB9178)
- SKU:
- CAB9178
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-ATP5L Antibody |
| Antibody SKU: | CAB9178 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human ATP5L (NP_006467.4). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | 293T, A-549, HepG2, HeLa, Mouse brain, Mouse liver, Rat brain, Rat kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-103 of human ATP5L (NP_006467.4). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAQF VRNL VEKT PALV NAAV TYSK PRLA TFWY YAKV ELVP PTPA EIPR AIQS LKKI VNSA QTGS FKQL TVKE AVLN GLVA TEVL MWFY VGEI IGKR GIIG YDV |
| Gene ID: | 10632 |
| Uniprot: | O75964 |
| Cellular Location: | Mitochondrion, Mitochondrion inner membrane |
| Calculated MW: | 11kDa |
| Observed MW: | 11kDa |
| Synonyms: | ATP5L, ATP5JG |
| Background: | Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the g subunit of the Fo complex. Alternative splicing results in multiple transcript variants. |
| UniProt Protein Function: | ATP5L: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(0) domain. Minor subunit located with subunit a in the membrane. Belongs to the ATPase g subunit family. |
| UniProt Protein Details: | Protein type:Transporter; Energy Metabolism - oxidative phosphorylation; Hydrolase; Membrane protein, integral; EC 3.6.3.14; Mitochondrial Chromosomal Location of Human Ortholog: 11q23.3 Cellular Component: mitochondrion; mitochondrial inner membrane; mitochondrial proton-transporting ATP synthase complex Molecular Function:ATPase activity; hydrogen ion transmembrane transporter activity; transmembrane transporter activity Biological Process: cellular metabolic process; mitochondrial ATP synthesis coupled proton transport |
| NCBI Summary: | Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel. The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3. The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene encodes the g subunit of the Fo complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jun 2010] |
| UniProt Code: | O75964 |
| NCBI GenInfo Identifier: | 51479156 |
| NCBI Gene ID: | 10632 |
| NCBI Accession: | NP_006467.4 |
| UniProt Secondary Accession: | O75964,Q96BV6, Q9UBZ7, A8K0K3, |
| UniProt Related Accession: | O75964 |
| Molecular Weight: | 11,428 Da |
| NCBI Full Name: | ATP synthase subunit g, mitochondrial |
| NCBI Synonym Full Names: | ATP synthase, H+ transporting, mitochondrial Fo complex, subunit G |
| NCBI Official Symbol: | ATP5L |
| NCBI Official Synonym Symbols: | ATP5JG |
| NCBI Protein Information: | ATP synthase subunit g, mitochondrial; ATPase subunit G; F1F0-type ATP synthase subunit g; ATP synthase g chain, mitochondrial; F1Fo-ATP synthase complex Fo membrane domain g subunit; ATP synthase, H+ transporting, mitochondrial F1F0, subunit g; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit G |
| UniProt Protein Name: | ATP synthase subunit g, mitochondrial |
| Protein Family: | ATP synthase |
| UniProt Gene Name: | ATP5L |
| UniProt Entry Name: | ATP5L_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATP5L antibody (CAB9178) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
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