Anti-ATP2A1 Antibody (CAB10104)

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SKU:
CAB10104
Product type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology
Frequently bought together:

Description

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Antibody Name:Anti-ATP2A1 Antibody
Antibody SKU:CAB10104
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 350-600 of human ATP2A1 (NP_775293.1).
Application:WB
Recommended Dilution:WB 1:200 - 1:1000
Reactivity:Human
Positive Samples:HL-60
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 350-600 of human ATP2A1 (NP_775293.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:SDKT GTLT TNQM SVCK MFII DKVD GDIC LLNE FSIT GSTY APEG EVLK NDKP VRPG QYDG LVEL ATIC ALCN DSSL DFNE AKGV YEKV GEAT ETAL TTLV EKMN VFNT DVRS LSKV ERAN ACNS VIRQ LMKK EFTL EFSR DRKS MSVY CSPA KSSR AAVG NKMF VKGA PEGV IDRC NYVR VGTT RVPL TGPV KEKI MAVI KEWG TGRD TLRC LALA TRDT PPKR EEMV LDDS ARFL EYET DLTF VGVV GML
Gene ID:487
Uniprot:O14983
Cellular Location:Endoplasmic reticulum membrane, Multi-pass membrane protein, Sarcoplasmic reticulum membrane
Calculated MW:95kDa/109kDa/110kDa
Observed MW:110kDa
Synonyms:ATP2A1, ATP2A, SERCA1
Background:This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms.
UniProt Protein Function:ATP2A1: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Associated with sarcolipin (SLN) and phospholamban (PLN). Increased contractile activity leads to a decrease in SERCA1 expression, while decreased contractile activity leads to an increase in SERCA1 expression. Skeletal muscle, fast twitch muscle (type II) fibers. Reversibly inhibited by phospholamban (PLN) at low calcium concentrations. Dephosphorylated PLN decreases the apparent affinity of the ATPase for calcium. This inhibition is regulated by the phosphorylation of PLN. Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transporter; Transporter, ion channel; Hydrolase; Membrane protein, multi-pass; Endoplasmic reticulum; Membrane protein, integral; EC 3.6.3.8

Chromosomal Location of Human Ortholog: 16p12.1

Cellular Component: I band; endoplasmic reticulum membrane; sarcoplasmic reticulum membrane; membrane; sarcoplasmic reticulum; perinuclear region of cytoplasm; ER-Golgi intermediate compartment; integral to membrane

Molecular Function:protein binding; protein homodimerization activity; calcium-transporting ATPase activity; calcium ion binding; ATP binding

Biological Process: apoptotic mitochondrial changes; metabolic process; positive regulation of fast-twitch skeletal muscle contraction; maintenance of mitochondrion localization; elevation of endoplasmic reticulum calcium ion concentration; reduction of endoplasmic reticulum calcium ion concentration; calcium ion transport; regulation of striated muscle contraction; blood coagulation; transmembrane transport; elevation of mitochondrial calcium ion concentration; negative regulation of striated muscle contraction

Disease: Brody Myopathy

NCBI Summary:This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction. Mutations in this gene cause some autosomal recessive forms of Brody disease, characterized by increasing impairment of muscular relaxation during exercise. Alternative splicing results in three transcript variants encoding different isoforms. [provided by RefSeq, Oct 2013]
UniProt Code:O14983
NCBI GenInfo Identifier:12643544
NCBI Gene ID:487
NCBI Accession:O14983.1
UniProt Secondary Accession:O14983,O14984, A8K5J9, B3KY17,
UniProt Related Accession:O14983
Molecular Weight:95,199 Da
NCBI Full Name:Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
NCBI Synonym Full Names:ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
NCBI Official Symbol:ATP2A1  
NCBI Official Synonym Symbols:ATP2A; SERCA1  
NCBI Protein Information:sarcoplasmic/endoplasmic reticulum calcium ATPase 1; calcium pump 1; SR Ca(2+)-ATPase 1; endoplasmic reticulum class 1/2 Ca(2+) ATPase; calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform
UniProt Protein Name:Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
UniProt Synonym Protein Names:Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletal muscle isoform; Endoplasmic reticulum class 1/2 Ca(2+) ATPase
Protein Family:Sarcoplasmic/endoplasmic reticulum calcium ATPase
UniProt Gene Name:ATP2A1  
UniProt Entry Name:AT2A1_HUMAN
Anti-ATP2A1 Antibody (CAB10104)Western blot analysis of extracts of HL-60 cells, using ATP2A1 antibody (CAB10104) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s.

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