Anti-ATP1B1 Antibody (CAB5793)
- SKU:
- CAB5793
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ATP1B1 Antibody |
| Antibody SKU: | CAB5793 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 63-303 of human ATP1B1 (NP_001668.1). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | HepG2, A-549, U-251MG |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 63-303 of human ATP1B1 (NP_001668.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | EFKP TYQD RVAP PGLT QIPQ IQKT EISF RPND PKSY EAYV LNIV RFLE KYKD SAQR DDMI FEDC GDVP SEPK ERGD FNHE RGER KVCR FKLE WLGN CSGL NDET YGYK EGKP CIII KLNR VLGF KPKP PKNE SLET YPVM KYNP NVLP VQCT GKRD EDKD KVGN VEYF GLGN SPGF PLQY YPYY GKLL QPKY LQPL LAVQ FTNL TMDT EIRI ECKA YGEN IGYS EKDR FQGR FDVK IEVK S |
| Gene ID: | 481 |
| Uniprot: | P05026 |
| Cellular Location: | Cell membrane, Single-pass type II membrane protein |
| Calculated MW: | 34kDa/35kDa |
| Observed MW: | 55kDa |
| Synonyms: | ATP1B1, ATP1B |
| Background: | The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. |
| UniProt Protein Function: | ATP1B1: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na(+) and K(+) ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. Belongs to the X(+)/potassium ATPases subunit beta family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transporter; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q24 Cellular Component: apical plasma membrane; basolateral plasma membrane; caveola; intracellular; membrane; myelin sheath; plasma membrane; sarcolemma; sodium:potassium-exchanging ATPase complex Molecular Function:ATP binding; ATPase activator activity; ATPase activity; ATPase binding; drug binding; potassium ion binding; protein binding; protein C-terminus binding; sodium ion binding; sodium:potassium-exchanging ATPase activity Biological Process: ATP metabolic process; blood coagulation; cardiac muscle contraction; cell adhesion; cellular calcium ion homeostasis; cellular potassium ion homeostasis; cellular sodium ion homeostasis; establishment and/or maintenance of transmembrane electrochemical gradient; leukocyte migration; positive regulation of ATPase activity; positive regulation of defense response to virus by host; potassium ion import; protein stabilization; regulation of gene expression; response to hypoxia; transmembrane transport Disease: Hypertension, Essential |
| NCBI Summary: | The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 1 subunit. Alternatively spliced transcript variants encoding different isoforms have been described, but their biological validity is not known. [provided by RefSeq, Mar 2010] |
| UniProt Code: | P05026 |
| NCBI GenInfo Identifier: | 114392 |
| NCBI Gene ID: | 481 |
| NCBI Accession: | P05026.1 |
| UniProt Secondary Accession: | P05026,Q5TGZ3, |
| UniProt Related Accession: | P05026 |
| Molecular Weight: | 34,893 Da |
| NCBI Full Name: | Sodium/potassium-transporting ATPase subunit beta-1 |
| NCBI Synonym Full Names: | ATPase Na+/K+ transporting subunit beta 1 |
| NCBI Official Symbol: | ATP1B1 |
| NCBI Official Synonym Symbols: | ATP1B |
| NCBI Protein Information: | sodium/potassium-transporting ATPase subunit beta-1 |
| UniProt Protein Name: | Sodium/potassium-transporting ATPase subunit beta-1 |
| UniProt Synonym Protein Names: | Sodium/potassium-dependent ATPase subunit beta-1 |
| UniProt Gene Name: | ATP1B1 |
| UniProt Entry Name: | AT1B1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATP1B1 antibody (CAB5793) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunohistochemistry of paraffin-embedded mouse stomach using ATP1B1 antibody (CAB5793) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human colon carcinoma using ATP1B1 antibody (CAB5793) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using ATP1B1 antibody (CAB5793) at dilution of 1:100 (40x lens). |
Related Products
Anti-ATP1B1 Antibody (CAB12403)
