Anti-ATGL/PNPLA2 Antibody (CAB6245)
- SKU:
- CAB6245
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Frequently bought together:
Description
| Antibody Name: | Anti-ATGL/PNPLA2 Antibody |
| Antibody SKU: | CAB6245 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human PNPLA2 (NP_065109.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-549, HepG2, THP-1, A673 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human PNPLA2 (NP_065109.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | PPAR PHGP EDKD QAVE SAQA EDYS QLPG EDHI LEHL PARL NEAL LEAC VEPT DLLT TLSN MLPV RLAT AMMV PYTL PLES ALSF TIRL LEWL PDVP EDIR WMKE QTGS ICQY LVMR AKRK LGRH LPSR LPEQ VELR RVQS LPSV PLSC AAYR EALP GWMR NNLS LGDA LAKW EECQ RQLL LGLF CTNV AFPP EALR MRAP ADPA PAPA DPAS PQHQ LAGP APLL STPA PEAR PVIG ALGL |
| Gene ID: | 57104 |
| Uniprot: | Q96AD5 |
| Cellular Location: | Cell membrane, Lipid droplet, Single-pass type II membrane protein |
| Calculated MW: | 19kDa/55kDa |
| Observed MW: | 55kDa |
| Synonyms: | PNPLA2, 1110001C14Rik, ATGL, FP17548, PEDF-R, TTS-2.2, TTS2, iPLA2zeta |
| Background: | This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. |
| UniProt Protein Function: | Catalyzes the initial step in triglyceride hydrolysis in adipocyte and non-adipocyte lipid droplets (PubMed:15550674). Also has acylglycerol transacylase activity. May act coordinately with LIPE/HLS within the lipolytic cascade. Regulates adiposome size and may be involved in the degradation of adiposomes (PubMed:16239926). May play an important role in energy homeostasis. May play a role in the response of the organism to starvation, enhancing hydrolysis of triglycerides and providing free fatty acids to other tissues to be oxidized in situations of energy depletion. |
| NCBI Summary: | This gene encodes an enzyme which catalyzes the first step in the hydrolysis of triglycerides in adipose tissue. Mutations in this gene are associated with neutral lipid storage disease with myopathy. [provided by RefSeq, Jul 2010] |
| UniProt Code: | Q96AD5 |
| NCBI GenInfo Identifier: | 74731110 |
| NCBI Gene ID: | 57104 |
| NCBI Accession: | Q96AD5.1 |
| UniProt Secondary Accession: | Q96AD5,O60643, Q5EFF5, Q6XYE5, Q96ET6, Q9NQ61, Q9NQ62 |
| UniProt Related Accession: | Q96AD5 |
| Molecular Weight: | 19,875 Da |
| NCBI Full Name: | Patatin-like phospholipase domain-containing protein 2 |
| NCBI Synonym Full Names: | patatin like phospholipase domain containing 2 |
| NCBI Official Symbol: | PNPLA2 |
| NCBI Official Synonym Symbols: | ATGL; TTS2; PEDF-R; FP17548; TTS-2.2; iPLA2zeta; 1110001C14Rik |
| NCBI Protein Information: | patatin-like phospholipase domain-containing protein 2 |
| UniProt Protein Name: | Patatin-like phospholipase domain-containing protein 2 |
| UniProt Synonym Protein Names: | Adipose triglyceride lipase; Calcium-independent phospholipase A2; Desnutrin; IPLA2-zeta; Pigment epithelium-derived factor; TTS2.2; Transport-secretion protein 2; TTS2 |
| UniProt Gene Name: | PNPLA2 |
 | Western blot analysis of extracts of various cell lines, using ATGL/PNPLA2 antibody (CAB6245) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
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