Anti-ATG3 Antibody (CAB5809)
- SKU:
- CAB5809
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-ATG3 Antibody |
| Antibody SKU: | CAB5809 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human ATG3 (NP_071933.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, HeLa, Mouse liver, Mouse heart, Mouse skeletal muscle, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-314 of human ATG3 (NP_071933.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MQNV INTV KGKA LEVA EYLT PVLK ESKF KETG VITP EEFV AAGD HLVH HCPT WQWA TGEE LKVK AYLP TGKQ FLVT KNVP CYKR CKQM EYSD ELEA IIEE DDGD GGWV DTYH NTGI TGIT EAVK EITL ENKD NIRL QDCS ALCE EEED EDEG EAAD MEEY EESG LLET DEAT LDTR KIVE ACKA KTDA GGED AILQ TRTY DLYI TYDK YYQT PRLW LFGY DEQR QPLT VEHM YEDI SQDH VKKT VTIE NHPH LPPP PMCS VHPC RHAE VMKK IIET VAEG GGEL GVHM YLLI FLKF VQAV IPTI EYDY TRHF TM |
| Gene ID: | 64422 |
| Uniprot: | Q9NT62 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 35kDa |
| Observed MW: | 37kDa |
| Synonyms: | ATG3, APG3, APG3-LIKE, APG3L, PC3-96 |
| Background: | This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | ATG3: an E2-like enzyme essential for Apg8p lipidation. |
| UniProt Protein Details: | Protein type:Ubiquitin conjugating system; Enzyme, cellular metabolism; Autophagy; EC 6.3.2.- Chromosomal Location of Human Ortholog: 3q13.2 Cellular Component: cytoplasmic ubiquitin ligase complex; cytosol Molecular Function:APG12 conjugating enzyme activity; APG8 conjugating enzyme activity; enzyme binding; protein binding; small conjugating protein ligase activity Biological Process: autophagic vacuole formation; macroautophagy; mitochondrial fragmentation during apoptosis; mitochondrion degradation; protein modification process; protein targeting to membrane; protein ubiquitination |
| NCBI Summary: | This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013] |
| UniProt Code: | Q9NT62 |
| NCBI GenInfo Identifier: | 61212142 |
| NCBI Gene ID: | 64422 |
| NCBI Accession: | Q9NT62.1 |
| UniProt Secondary Accession: | Q9NT62,Q6PKC5, Q9H6L9, |
| UniProt Related Accession: | Q9NT62 |
| Molecular Weight: | 35,467 Da |
| NCBI Full Name: | Ubiquitin-like-conjugating enzyme ATG3 |
| NCBI Synonym Full Names: | autophagy related 3 |
| NCBI Official Symbol: | ATG3 |
| NCBI Official Synonym Symbols: | APG3; APG3L; PC3-96; APG3-LIKE |
| NCBI Protein Information: | ubiquitin-like-conjugating enzyme ATG3 |
| UniProt Protein Name: | Ubiquitin-like-conjugating enzyme ATG3 |
| UniProt Synonym Protein Names: | Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96 |
| Protein Family: | Autophagy-related protein |
| UniProt Gene Name: | ATG3 |
| UniProt Entry Name: | ATG3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATG3 antibody (CAB5809) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded mouse lung using ATG3 antibody (CAB5809) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse spleen using ATG3 antibody (CAB5809) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of NIH-3T3 cells using ATG3 Rabbit pAb (CAB5809) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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