Anti-ATG16L1 Antibody (CAB1871)
- SKU:
- CAB1871
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Autophagy
Frequently bought together:
Description
| Antibody Name: | Anti-ATG16L1 Antibody |
| Antibody SKU: | CAB1871 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-300 of human ATG16L1 (NP_110430.5). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-937, 293T, HepG2, SH-SY5Y, SW620 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 90-300 of human ATG16L1 (NP_110430.5). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IKHQ EELT ELHK KRGE LAQL VIDL NNQM QRKD REMQ MNEA KIAE CLQT ISDL ETEC LDLR TKLC DLER ANQT LKDE YDAL QITF TALE GKLR KTTE ENQE LVTR WMAE KAQE ANRL NAEN EKDS RRRQ ARLQ KELA EAAK EPLP VEQD DDIE VIVD ETSD HTEE TSPV RAIS RAAT KRLS QPAG GLLD SITN IFGR RSVS SFPV PQDN VDT |
| Gene ID: | 55054 |
| Uniprot: | Q676U5 |
| Cellular Location: | Cytoplasm, Peripheral membrane protein, Preautophagosomal structure membrane |
| Calculated MW: | 47kDa/49kDa/53kDa/66kDa/68kDa |
| Observed MW: | 68kDa |
| Synonyms: | ATG16L1, APG16L, ATG16A, ATG16L, IBD10, WDR30 |
| Background: | The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene. |
| UniProt Protein Function: | ATG16L1: Plays an essential role in autophagy. Homooligomer. Interacts with ATG5. Part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kDa) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kDa). Belongs to the WD repeat ATG16 family. 5 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Membrane protein, peripheral; Adaptor/scaffold; Autophagy Chromosomal Location of Human Ortholog: 2q37.1 Cellular Component: autophagic vacuole; axoneme; cytosol Molecular Function:identical protein binding; protein binding; small conjugating protein ligase activity Biological Process: autophagic vacuole formation; macroautophagy Disease: Inflammatory Bowel Disease 10 |
| NCBI Summary: | The protein encoded by this gene is part of a large protein complex that is necessary for autophagy, the major process by which intracellular components are targeted to lysosomes for degradation. Defects in this gene are a cause of susceptibility to inflammatory bowel disease type 10 (IBD10). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2010] |
| UniProt Code: | Q676U5 |
| NCBI GenInfo Identifier: | 62510482 |
| NCBI Gene ID: | 55054 |
| NCBI Accession: | Q676U5.2 |
| UniProt Secondary Accession: | Q676U5,Q6IPN1, Q6UXW4, Q6ZVZ5, Q8NCY2, Q96JV5, Q9H619 A3EXK9, A3EXL0, B6ZDH0, |
| UniProt Related Accession: | Q676U5 |
| Molecular Weight: | 49,486 Da |
| NCBI Full Name: | Autophagy-related protein 16-1 |
| NCBI Synonym Full Names: | autophagy related 16 like 1 |
| NCBI Official Symbol: | ATG16L1 |
| NCBI Official Synonym Symbols: | IBD10; WDR30; APG16L; ATG16A; ATG16L |
| NCBI Protein Information: | autophagy-related protein 16-1 |
| UniProt Protein Name: | Autophagy-related protein 16-1 |
| UniProt Synonym Protein Names: | APG16-like 1 |
| Protein Family: | Autophagy-related protein |
| UniProt Gene Name: | ATG16L1 |
| UniProt Entry Name: | A16L1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ATG16L1 antibody (CAB1871) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunofluorescence analysis of A-549 cells using ATG16L1 antibody (CAB1871). Blue: DAPI for nuclear staining. |
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