Anti-ASZ1 Antibody (CAB14447)
- SKU:
- CAB14447
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Frequently bought together:
Description
| Antibody Name: | Anti-ASZ1 Antibody |
| Antibody SKU: | CAB14447 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human ASZ1 (NP_570124.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Mouse testis, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-350 of human ASZ1 (NP_570124.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAAS ALRG LPVA GGGE SSES EDDG WEIG YLDR TSQK LKRL LPIE EKKE KFKK AMTI GDVS LVQE LLDS GISV DSNF QYGW TPLM YAAS VANA ELVR VLLD RGAN ASFE KDKQ SILI TACS AHGS EEQI LKCV ELLL SRNA DPNV ACRR LMTP IMYA ARDG HTQV VALL VAHG AEVN TQDE NGYT ALTW AARQ GHKN IVLK LLEL GANK MLQT KDGK MPSE IAKR NKHH EIFN LLSF TLNP LEGK LQQL TKED TICK ILTT DSDR EKDH IFSS YTAF GDLE VFLH GLGL EHMT DLLK ERDI TLRH LLTM REDE FTKN GITS KDQQ KILA ALKE LQVE EIQF GELS EETK LE |
| Gene ID: | 136991 |
| Uniprot: | Q8WWH4 |
| Cellular Location: | Cytoplasm |
| Calculated MW: | 52kDa/53kDa |
| Observed MW: | 53kDa |
| Synonyms: | ASZ1, ALP1, ANKL1, C7orf7, CT1.19, GASZ, Orf3 |
| Background: |
| UniProt Protein Function: | ASZ1: Plays a central role during spermatogenesis by repressing transposable elements and prevent their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Its association with pi-bodies suggests a participation in the primary piRNAs metabolic process. Required prior to the pachytene stage to facilitate the production of multiple types of piRNAs, including those associated with repeats involved in regulation of retrotransposons. May act by mediating protein-protein interactions during germ cell maturation. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 7q31.2 Cellular Component: cytoplasm Molecular Function:signal transducer activity Biological Process: DNA methylation during gametogenesis; multicellular organismal development; RNA-mediated gene silencing; spermatogenesis; gene expression; male meiosis; signal transduction; cell differentiation |
| UniProt Code: | Q8WWH4 |
| NCBI GenInfo Identifier: | 18640738 |
| NCBI Gene ID: | 136991 |
| NCBI Accession: | NP_570124.1 |
| UniProt Secondary Accession: | Q8WWH4,A0AV27, A4D0V0, |
| UniProt Related Accession: | Q8WWH4 |
| Molecular Weight: | 53,458 Da |
| NCBI Full Name: | ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1 isoform 1 |
| NCBI Synonym Full Names: | ankyrin repeat, SAM and basic leucine zipper domain containing 1 |
| NCBI Official Symbol: | ASZ1 |
| NCBI Official Synonym Symbols: | ALP1; GASZ; Orf3; ANKL1; C7orf7; CT1.19 |
| NCBI Protein Information: | ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1; ankyrin-like 1; ankyrin-like protein 1; germ cell-specific ankyrin, SAM and basic leucine zipper domain-containing protein |
| UniProt Protein Name: | Ankyrin repeat, SAM and basic leucine zipper domain-containing protein 1 |
| UniProt Synonym Protein Names: | Ankyrin-like protein 1; Germ cell-specific ankyrin, SAM and basic leucine zipper domain-containing protein |
| Protein Family: | Ankyrin repeat, SAM and basic leucine zipper domain-containing protein |
| UniProt Gene Name: | ASZ1 |
| UniProt Entry Name: | ASZ1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ASZ1 antibody (CAB14447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat testis using ASZ1 antibody (CAB14447) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse testis using ASZ1 antibody (CAB14447) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of rat testis cells using ASZ1 antibody (CAB14447) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of mouse testis cells using ASZ1 antibody (CAB14447) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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