Anti-ASNS Antibody (CAB16035)
- SKU:
- CAB16035
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Metabolism
Description
| Antibody Name: | Anti-ASNS Antibody |
| Antibody SKU: | CAB16035 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 262-561 of human ASNS (NP_597680.2). |
| Application: | WB IHC |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SGC-7901, U-87MG, Mouse brain, Mouse pancreas, Mouse spleen, Rat testis |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 262-561 of human ASNS (NP_597680.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | DSSL VAAT LLKQ LKEA QVQY PLQT FAIG MEDS PDLL AARK VADH IGSE HYEV LFNS EEGI QALD EVIF SLET YDIT TVRA SVGM YLIS KYIR KNTD SVVI FSGE GSDE LTQG YIYF HKAP SPEK AEEE SERL LREL YLFD VLRA DRTT AAHG LELR VPFL DHRF SSYY LSLP PEMR IPKN GIEK HLLR ETFE DSNL IPKE ILWR PKEA FSDG ITSV KNSW FKIL QEYV EHQV DDAM MANA AQKF PFNT PKTK EGYY YRQV FERH YPGR ADWL SHYW MPKW INAT DPSA RTLT HYKS AVKA |
| Gene ID: | 440 |
| Uniprot: | P08243 |
| Cellular Location: | |
| Calculated MW: | 54kDa/62kDa/64kDa |
| Observed MW: | 55, 64kDa |
| Synonyms: | ASNS, ASNSD, TS11 |
| Background: | The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. |
| UniProt Protein Function: | ASNS: 3 isoforms of the human protein are produced by alternative splicing |
| UniProt Protein Details: | Protein type:Amino Acid Metabolism - alanine, aspartate and glutamate; Cell cycle regulation; Ligase; Energy Metabolism - nitrogen; EC 6.3.5.4 Chromosomal Location of Human Ortholog: 7q21.3 Cellular Component: cytosol Molecular Function:protein homodimerization activity; asparagine synthase (glutamine-hydrolyzing) activity; cofactor binding; ATP binding Biological Process: response to light stimulus; unfolded protein response; response to toxin; glutamine metabolic process; liver development; positive regulation of mitotic cell cycle; response to amino acid stimulus; cellular response to hormone stimulus; cellular response to glucose starvation; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; asparagine biosynthetic process; response to mechanical stimulus; response to methotrexate; amino acid biosynthetic process; response to follicle-stimulating hormone stimulus; negative regulation of apoptosis Disease: Asparagine Synthetase Deficiency |
| NCBI Summary: | The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010] |
| UniProt Code: | P08243 |
| NCBI GenInfo Identifier: | 13432102 |
| NCBI Gene ID: | 440 |
| NCBI Accession: | P08243.4 |
| UniProt Secondary Accession: | P08243,P08184, Q15666, Q549T9, Q96HD0, A4D1I8, B4DXZ1 B7ZAA9, D6W5R3, E9PCI3, E9PCX6, |
| UniProt Related Accession: | P08243 |
| Molecular Weight: | 561 |
| NCBI Full Name: | Asparagine synthetase |
| NCBI Synonym Full Names: | asparagine synthetase (glutamine-hydrolyzing) |
| NCBI Official Symbol: | ASNS |
| NCBI Official Synonym Symbols: | TS11; ASNSD |
| NCBI Protein Information: | asparagine synthetase [glutamine-hydrolyzing]; asparagine synthetase [glutamine-hydrolyzing]; TS11 cell cycle control protein; glutamine-dependent asparagine synthetase |
| UniProt Protein Name: | Asparagine synthetase [glutamine-hydrolyzing] |
| UniProt Synonym Protein Names: | Cell cycle control protein TS11; Glutamine-dependent asparagine synthetase |
| Protein Family: | Asparagine--tRNA ligase |
| UniProt Gene Name: | ASNS |
| UniProt Entry Name: | ASNS_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ASNS antibody (CAB16035) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunohistochemistry of paraffin-embedded rat ovary using ASNS antibody (CAB16035) at dilution of 1:100 (40x lens). |
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