Anti-ASF1B 항체 (CAB6511)

Antibody Name:	Anti-ASF1B Antibody
Antibody SKU:	CAB6511
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 63-202 of human ASF1B (NP_060624.1).

Application:	WB
Recommended Dilution:	WB 1:200 - 1:1000
Reactivity:	Human
Positive Samples:	HeLa, MCF7, THP-1

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 63-202 of human ASF1B (NP_060624.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	GPVP AGRH MFVF QADA PNPS LIPE TDAV GVTV VLIT CTYH GQEF IRVG YYVN NEYL NPEL RENP PMKP DFSQ LQRN ILAS NPRV TRFH INWD NNMD RLEA IETQ DPSL GCGL PLNC TPIK GLGL PGCI PGLL PENS MDCI
Gene ID:	55723
Uniprot:	Q9NVP2
Cellular Location:	Nucleus
Calculated MW:	22kDa
Observed MW:	17kDa

Synonyms:	ASF1B, CIA-II
Background:	This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly.

UniProt Protein Function:	ASF1B: Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis. Interacts with histone H3 (including both histone H3.1 and H3.3) and histone H4. Interacts with the CHAF1A, CHAF1B and RBBP4 subunits of the CAF-1 complex. Interacts with HAT1, NASP, TAF1, TLK1 and TLK2. Highly expressed in testis and at lower levels in colon, small intestine and thymus. Belongs to the ASF1 family.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 19p13.12 Cellular Component: nuclear chromatin; nucleoplasm; protein complex Molecular Function:histone binding; protein binding Biological Process: cell differentiation; chromatin modification; DNA replication-dependent nucleosome assembly; DNA replication-independent nucleosome assembly; multicellular organismal development; regulation of transcription, DNA-dependent; spermatogenesis; transcription, DNA-dependent
NCBI Summary:	This gene encodes a member of the H3/H4 family of histone chaperone proteins and is similar to the anti-silencing function-1 gene in yeast. The encoded protein is the substrate of the tousled-like kinase family of cell cycle-regulated kinases, and may play a key role in modulating the nucleosome structure of chromatin by ensuring a constant supply of histones at sites of nucleosome assembly. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9NVP2
NCBI GenInfo Identifier:	74734533
NCBI Gene ID:	55723
NCBI Accession:	Q9NVP2.1
UniProt Secondary Accession:	Q9NVP2,Q53G51, Q9NVZ0,
UniProt Related Accession:	Q9NVP2
Molecular Weight:	22,434 Da
NCBI Full Name:	Histone chaperone ASF1B
NCBI Synonym Full Names:	anti-silencing function 1B histone chaperone
NCBI Official Symbol:	ASF1B
NCBI Official Synonym Symbols:	CIA-II
NCBI Protein Information:	histone chaperone ASF1B
UniProt Protein Name:	Histone chaperone ASF1B
UniProt Synonym Protein Names:	Anti-silencing function protein 1 homolog B; hAsf1; hAsf1b; CCG1-interacting factor A-II; CIA-II; hCIA-II
Protein Family:	Histone chaperone
UniProt Gene Name:	ASF1B
UniProt Entry Name:	ASF1B_HUMAN

Western blot analysis of extracts of various cell lines, using ASF1B antibody (CAB6511) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.

Antibodies

ASF1B Antibody (PACO04716)

ASF1B Antibody (PACO07881)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)