Anti-ARL8B Antibody (CAB8869)
- SKU:
- CAB8869
- Product type:
- Antibody
- Application:
- WB
- Reactivity:
- Human
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-ARL8B Antibody |
| Antibody SKU: | CAB8869 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 127-186 of human ARL8B (NP_060654.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:3000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse |
| Positive Samples: | U-87MG, LO2, Mouse brain, Mouse heart, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 127-186 of human ARL8B (NP_060654.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VLGN KRDL PNAL DEKQ LIEK MNLS AIQD REIC CYSI SCKE KDNI DITL QWLI QHSK SRRS |
| Gene ID: | 55207 |
| Uniprot: | Q9NVJ2 |
| Cellular Location: | Cytoplasm, Late endosome membrane, Lysosome membrane, cytoskeleton, spindle |
| Calculated MW: | 18kDa/21kDa |
| Observed MW: | 21kDa |
| Synonyms: | ARL8B, ARL10C, Gie1 |
| Background: |
| UniProt Protein Function: | ARL8B: May play a role in lysosome motility (PubMed:16537643). May play a role in chromosome segregation (PubMed:15331635). Belongs to the small GTPase superfamily. Arf family. |
| UniProt Protein Details: | Protein type:G protein, monomeric, ARF; G protein, monomeric Chromosomal Location of Human Ortholog: 3p26.1 Cellular Component: cytoplasm; late endosome membrane; lysosomal membrane; membrane; midbody; spindle midzone Molecular Function:alpha-tubulin binding; beta-tubulin binding; GDP binding; GTP binding; GTPase activity Biological Process: cell cycle; cell division; chromosome segregation; small GTPase mediated signal transduction |
| UniProt Code: | Q9NVJ2 |
| NCBI GenInfo Identifier: | 8922601 |
| NCBI Gene ID: | 55207 |
| NCBI Accession: | NP_060654.1 |
| UniProt Secondary Accession: | Q9NVJ2,B4DI85, |
| UniProt Related Accession: | Q9NVJ2 |
| Molecular Weight: | 18,642 Da |
| NCBI Full Name: | ADP-ribosylation factor-like protein 8B |
| NCBI Synonym Full Names: | ADP ribosylation factor like GTPase 8B |
| NCBI Official Symbol: | ARL8B |
| NCBI Official Synonym Symbols: | Gie1; ARL10C |
| NCBI Protein Information: | ADP-ribosylation factor-like protein 8B |
| UniProt Protein Name: | ADP-ribosylation factor-like protein 8B |
| UniProt Synonym Protein Names: | ADP-ribosylation factor-like protein 10C; Novel small G protein indispensable for equal chromosome segregation 1 |
| Protein Family: | ADP-ribosylation factor-like protein |
| UniProt Gene Name: | ARL8B |
| UniProt Entry Name: | ARL8B_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARL8B antibody (CAB8869) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s. |
 | Confocal immunofluorescence analysis of U2OS cells using Anti-ARL8B Polyclonal Antibody (CAB8869) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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