Anti-ARL3 Antibody (CAB16348)
- SKU:
- CAB16348
- Product type:
- Antibody
- Reactivity:
- Human
- Mouse
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Cycle
Frequently bought together:
Description
| Antibody Name: | Anti-ARL3 Antibody |
| Antibody SKU: | CAB16348 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human ARL3 (NP_004302.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | A-431, LO2, HeLa, Mouse eye, Mouse brain, Mouse heart, Mouse kidney, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-182 of human ARL3 (NP_004302.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGLL SILR KLKS APDQ EVRI LLLG LDNA GKTT LLKQ LASE DISH ITPT QGFN IKSV QSQG FKLN VWDI GGQR KIRP YWKN YFEN TDIL IYVI DSAD RKRF EETG QELA ELLE EEKL SCVP VLIF ANKQ DLLT AAPA SEIA EGLN LHTI RDRV WQIQ SCSA LTGE GVQD GMNW VCKN VNAK KK |
| Gene ID: | 403 |
| Uniprot: | P36405 |
| Cellular Location: | Cell projection, Cytoplasm, Cytoplasmic side, Golgi apparatus membrane, Nucleus, Peripheral membrane protein, centrosome, cilium, cytoskeleton, microtubule organizing center, spindle |
| Calculated MW: | 20kDa |
| Observed MW: | 20kDa |
| Synonyms: | ARL3, ARFL3 |
| Background: | ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. |
| UniProt Protein Function: | ARL3: Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). Required for normal cytokinesis and cilia signaling. Requires assistance from GTPase- activating proteins (GAPs) like RP2 and PDE6D, in order to cycle between inactive GDP-bound and active GTP-bound forms. Required for targeting proteins such as NPHP3 to the ciliary membrane by releasing myristoylated NPHP3 from UNC119B cargo adapter into the cilium. Does not act as an allosteric activator of the cholera toxin catalytic subunit. Belongs to the small GTPase superfamily. Arf family. |
| UniProt Protein Details: | Protein type:Cytoskeletal; Cell cycle regulation; G protein, monomeric; G protein, monomeric, ARF Chromosomal Location of Human Ortholog: 10q23.3 Cellular Component: Golgi membrane; Golgi apparatus; centrosome; cytoplasmic microtubule; spindle microtubule; midbody; nucleus; photoreceptor connecting cilium Molecular Function:GTPase activity; protein binding; GDP binding; GTP binding; microtubule binding; metal ion binding Biological Process: photoreceptor cell development; protein transport; smoothened signaling pathway; metabolic process; small GTPase mediated signal transduction; organelle organization and biogenesis; intraflagellar transport; cytokinesis; post-Golgi vesicle-mediated transport; kidney development |
| NCBI Summary: | ADP-ribosylation factor-like 3 is a member of the ADP-ribosylation factor family of GTP-binding proteins. ARL3 binds guanine nucleotides but lacks ADP-ribosylation factor activity. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P36405 |
| NCBI GenInfo Identifier: | 543851 |
| NCBI Gene ID: | 403 |
| NCBI Accession: | P36405.2 |
| UniProt Secondary Accession: | P36405,Q53X83, Q5JSM2, B2R6C7, |
| UniProt Related Accession: | P36405 |
| Molecular Weight: | 182 |
| NCBI Full Name: | ADP-ribosylation factor-like protein 3 |
| NCBI Synonym Full Names: | ADP-ribosylation factor-like 3 |
| NCBI Official Symbol: | ARL3 |
| NCBI Official Synonym Symbols: | ARFL3 |
| NCBI Protein Information: | ADP-ribosylation factor-like protein 3; ARF-like 3 |
| UniProt Protein Name: | ADP-ribosylation factor-like protein 3 |
| Protein Family: | ADP-ribosylation factor-like protein |
| UniProt Gene Name: | ARL3 |
| UniProt Entry Name: | ARL3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ARL3 antibody (CAB16348) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 cells using ARL3 Polyclonal Antibody (CAB16348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using ARL3 Polyclonal Antibody (CAB16348) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining. |
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